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PMID: 7919989 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Control of Arabidopsis flower and seed development by the homeotic gene APETALA2.

The Plant cell ·Vol. 6 ·No. 9 ·1994-09-00 ·Pages 1211-25

Jofuku KD, den Boer BG, Van Montagu M, Okamuro JK

Abstract

APETALA2 (AP2) plays a central role in the establishment of the floral meristem, the specification of floral organ identity, and the regulation of floral homeotic gene expression in Arabidopsis. We show here that in addition to its functions during flower development, AP2 activity is also required during seed development. We isolated the AP2 gene and found that it encodes a putative nuclear protein that is distinguished by an essential 68-amino acid repeated motif, the AP2 domain. Consistent with its genetic functions, we determined that AP2 is expressed at the RNA level in all four types of floral organs--sepals, petals, stamens, and carpels--and in developing ovules. Thus, AP2 gene transcription does not appear to be spatially restricted by the floral homeotic gene AGAMOUS as predicted by previous studies. We also found that AP2 is expressed at the RNA level in the inflorescence meristem and in nonfloral organs, including leaf and stem. Taken together, our results suggest that AP2 represents a new class of plant regulatory proteins that may play a general role in the control of Arabidopsis development.

Related Genes
APETALA2
MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Arabidopsis Proteins Base Sequence Gene Expression Regulation, Plant Genes, Homeobox Genes, Plant Homeodomain Proteins/genetics Molecular Sequence Data Nuclear Proteins/genetics Plant Proteins Seeds/growth & development
Chemicals
APETALA2 protein, Arabidopsis Arabidopsis Proteins Homeodomain Proteins Nuclear Proteins Plant Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jofuku K D
Sinsheimer Laboratories, Department of Biology, University of California, Santa Cruz 95064.
den Boer B G
Van Montagu M
Okamuro J K
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.6.9.1211
Published
1994-09-00
Pages
1211-25
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160514
Subset
IM
Grants
NIGMS NIH HHS · GM46309 · United States
NCRR NIH HHS · RR08132 · United States
Databases
GENBANK
U12546
External Links
PubMed source DOI: 10.1105/tpc.6.9.1211 PMC Full Text View journal details
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