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PMID: 2385959 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Hemagglutinating encephalomyelitis virus attaches to N-acetyl-9-O-acetylneuraminic acid-containing receptors on erythrocytes: comparison with bovine coronavirus and influenza C virus.

Virus research ·Vol. 16 ·No. 2 ·1990-06-00 ·Pages 185-94

Schultze B, Gross HJ, Brossmer R, Klenk HD, Herrler G

Abstract

The receptors for the hemagglutinating encephalomyelitis virus (HEV, a porcine coronavirus) on chicken erythrocytes were analyzed and compared to the receptors for bovine coronavirus (BCV) and influenza C virus. Evidence was obtained that HEV requires the presence of N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) on the cell surface for agglutination of erythrocytes as has been previously shown for BCV and influenza C virus: (i) Incubation of red blood cells with sialate 9-O-acetylesterase, the receptor-destroying enzyme of influenza C virus, rendered the erythrocytes resistant against agglutination by each of the three viruses; (ii) Human erythrocytes which are resistant to agglutination by HEV acquire receptors for HEV after resialylation with Neu5,9Ac2. Sialylation of red blood cells with limiting amounts of sialic acid indicated that strain JHB/1/66 of influenza C virus requires less Neu5,9Ac2 for agglutination of erythrocytes than the two coronaviruses, both of which were found to be similar in their reactivity with Neu5,9Ac2-containing receptors.

MeSH Terms
Acetylesterase Animals Carboxylic Ester Hydrolases/pharmacology Cells, Cultured Coronaviridae/drug effects,metabolism Erythrocytes/drug effects,metabolism Hemagglutination, Viral Humans Influenzavirus C/metabolism Orthomyxoviridae/metabolism Receptors, Virus/drug effects,metabolism Sialic Acids/metabolism
Chemicals
Receptors, Virus Sialic Acids 9-O-acetyl-N-acetylneuraminic acid Carboxylic Ester Hydrolases Acetylesterase sialate O-acetylesterase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schultze B
Institut für Virologie, Philipps-Universität Marburg, F.R.G.
Gross H J
Brossmer R
Klenk H D
Herrler G
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Article Info
Journal
Virus research
Abbr.
Virus Res
ISSN
0168-1702
DOI
10.1016/0168-1702(90)90022-4
Published
1990-06-00
Pages
185-94
Language
English
Region
Netherlands
NLM ID
8410979
PMCID
PMC7134004
Subset
IM
External Links
PubMed source DOI: 10.1016/0168-1702(90)90022-4 PMC Full Text View journal details
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