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PMID: 3184275 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The E3 protein of bovine coronavirus is a receptor-destroying enzyme with acetylesterase activity.

Journal of virology ·Vol. 62 ·No. 12 ·1988-12-00 ·Pages 4686-90

Vlasak R, Luytjes W, Leider J, Spaan W, Palese P

Abstract

In addition to members of the Orthomyxoviridae and Paramyxoviridae, several coronaviruses have been shown to possess receptor-destroying activities. Purified bovine coronavirus (BCV) preparations have an esterase activity which inactivates O-acetylsialic acid-containing receptors on erythrocytes. Diisopropyl fluorophosphate (DFP) completely inhibits this receptor-destroying activity of BCV, suggesting that the viral enzyme is a serine esterase. Treatment of purified BCV with [3H]DFP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the proteins revealed that the E3 protein was specifically phosphorylated. This finding suggests that the esterase/receptor-destroying activity of BCV is associated with the E3 protein. Furthermore, treatment of BCV with DFP dramatically reduced its infectivity in a plaque assay. It is assumed that the esterase activity of BCV is required in an early step of virus replication, possibly during virus entry or uncoating.

MeSH Terms
Acetylesterase/antagonists & inhibitors,metabolism Animals Cattle Cell Line Coronaviridae/drug effects,enzymology,physiology Glycoproteins/metabolism Hemagglutination Tests Hemagglutination, Viral Isoflurophate/pharmacology Receptors, Virus/metabolism Viral Plaque Assay Viral Proteins/metabolism Virus Replication
Chemicals
Glycoproteins Receptors, Virus Viral Proteins Isoflurophate Acetylesterase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vlasak R
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029-6574.
Luytjes W
Leider J
Spaan W
Palese P
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.12.4686-4690.1988
Published
1988-12-00
Pages
4686-90
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253582
Subset
IM
Grants
NIAID NIH HHS · AI-11823 · United States
NIAID NIH HHS · AI18998 · United States
NIGMS NIH HHS · GM07280 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.12.4686-4690.1988 PMC Full Text View journal details
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