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PMID: 23812720 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Elongation factor G bound to the ribosome in an intermediate state of translocation.

Science (New York, N.Y.) ·Vol. 340 ·No. 6140 ·2013-06-28 ·Pages 1235490

Tourigny DS, Fernández IS, Kelley AC, Ramakrishnan V

Abstract

A key step of translation by the ribosome is translocation, which involves the movement of messenger RNA (mRNA) and transfer RNA (tRNA) with respect to the ribosome. This allows a new round of protein chain elongation by placing the next mRNA codon in the A site of the 30S subunit. Translocation proceeds through an intermediate state in which the acceptor ends of the tRNAs have moved with respect to the 50S subunit but not the 30S subunit, to form hybrid states. The guanosine triphosphatase (GTPase) elongation factor G (EF-G) catalyzes the subsequent movement of mRNA and tRNA with respect to the 30S subunit. Here, we present a crystal structure at 3 angstrom resolution of the Thermus thermophilus ribosome with a tRNA in the hybrid P/E state bound to EF-G with a GTP analog. The structure provides insights into structural changes that facilitate translocation and suggests a common GTPase mechanism for EF-G and elongation factor Tu.

MeSH Terms
Amino Acid Sequence Catalytic Domain Crystallography, X-Ray Guanosine Triphosphate/analogs & derivatives Molecular Sequence Data Peptide Elongation Factor G/chemistry Protein Biosynthesis Protein Structure, Tertiary RNA, Messenger/chemistry RNA, Transfer/chemistry Ribosomes/chemistry Thermus thermophilus/enzymology
Chemicals
Peptide Elongation Factor G RNA, Messenger Guanosine Triphosphate RNA, Transfer
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tourigny David S
MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.
Fernández Israel S
Kelley Ann C
Ramakrishnan V
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1235490
Published
2013-06-28
Pages
1235490
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC3836249
Subset
IM
Grants
Wellcome Trust · 096570 · United Kingdom
Medical Research Council · MC_U105184332 · United Kingdom
Medical Research Council · U105184332 · United Kingdom
Databases
PDB
4JUW, 4JUX
Corrections
CommentIn
PMID: 23812707
External Links
PubMed source DOI: 10.1126/science.1235490 PMC Full Text View journal details
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