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PMID: 22459262 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Functional role of the sarcin-ricin loop of the 23S rRNA in the elongation cycle of protein synthesis.

Journal of molecular biology ·Vol. 419 ·No. 3-4 ·2012-06-08 ·Pages 125-38

Shi X, Khade PK, Sanbonmatsu KY, Joseph S

Abstract

The sarcin-ricin loop (SRL) is one of the longest conserved sequences in the 23S ribosomal RNA. The SRL has been accepted as crucial for the activity of the ribosome because it is targeted by cytotoxins such as α-sarcin and ricin that completely abolish translation. Nevertheless, the precise functional role of the SRL in translation is not known. Recent biochemical and structural studies indicate that the SRL is critical for triggering GTP hydrolysis on elongation factor Tu (EF-Tu) and elongation factor G (EF-G). To determine the functional role of the SRL in the elongation stage of protein synthesis, we analyzed mutations in the SRL that are known to abolish protein synthesis and are lethal to cells. Here, we show that the SRL is not critical for GTP hydrolysis on EF-Tu and EF-G. The SRL also is not essential for peptide bond formation. Our results, instead, suggest that the SRL is crucial for anchoring EF-G on the ribosome during mRNA-tRNA translocation.

MeSH Terms
Binding Sites Conserved Sequence Endoribonucleases/metabolism Escherichia coli/genetics Fungal Proteins/metabolism Guanosine Triphosphate/metabolism Mutation Nucleic Acid Conformation Peptide Chain Elongation, Translational Peptide Elongation Factor G/chemistry,genetics,metabolism Peptide Elongation Factor Tu/chemistry,genetics,metabolism Protein Binding Protein Biosynthesis Protein Structure, Secondary RNA, Bacterial/chemistry,genetics,metabolism RNA, Messenger/metabolism RNA, Ribosomal/genetics,metabolism RNA, Ribosomal, 23S/chemistry,genetics,metabolism RNA, Transfer/metabolism Ribosomes/genetics,metabolism Ricin/metabolism
Chemicals
Fungal Proteins Peptide Elongation Factor G RNA, Bacterial RNA, Messenger RNA, Ribosomal RNA, Ribosomal, 23S alpha-sarcin Guanosine Triphosphate Ricin RNA, Transfer Endoribonucleases Peptide Elongation Factor Tu
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shi Xinying
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0314, USA.
Khade Prashant K
Sanbonmatsu Karissa Y
Joseph Simpson
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Article Info
Journal
Journal of molecular biology
Abbr.
J Mol Biol
ISSN
1089-8638
DOI
10.1016/j.jmb.2012.03.016
Published
2012-06-08
Epub
2012-00-26
Pages
125-38
Language
English
Region
England
NLM ID
2985088R
PMCID
PMC3348345
Subset
IM
Grants
NIGMS NIH HHS · R01 GM065265 · United States
NIGMS NIH HHS · R01 GM065265-09 · United States
NIGMS NIH HHS · R01 GM072686 · United States
NIGMS NIH HHS · GM065265 · United States
Databases
PDB
2XQE
External Links
PubMed source DOI: 10.1016/j.jmb.2012.03.016 PMC Full Text View journal details
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