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PMID: 2367536 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning of a member of a third class of Shaker-family K+ channel genes in mammals.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 13 ·1990-07-00 ·Pages 5227-31

McCormack T, Vega-Saenz de Miera EC, Rudy B

Abstract

We report the cloning of RKShIIIA, a cDNA encoding a K+ channel sequence expressed in rat brain. This cDNA encodes K+ channel subunits that express in Xenopus oocytes a slow, 4-aminopyridine- and tetraethylammonium-sensitive, delayed rectifier-type K+ channel activated by large membrane depolarizations. This gene belongs to the Shaker (Sh) family of K+ channel genes, since the predicted protein has the same overall structure and shows significant homology to other members of this family. However, RKShIIIA cannot be assigned to either of the two known classes of Sh-family genes in mammals based on sequence analysis. Notable features of the RKShIIIA protein product include a probable cytoplasmic loop rich in prolines and a stretch very homologous to the Drosophila Shaw protein, both near the amino terminus.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular/methods Drosophila/genetics Electric Conductivity Female Humans Membrane Potentials Membrane Proteins/genetics,physiology Microinjections Molecular Sequence Data Multigene Family Oocytes/physiology Potassium Channels/physiology RNA, Messenger/administration & dosage,genetics Rats Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Membrane Proteins Potassium Channels RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McCormack T
Department of Physiology and Biophysics, New York University Medical Center, NY 10016.
Vega-Saenz de Miera E C
Rudy B
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.13.5227
Published
1990-07-00
Pages
5227-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54295
Subset
IM
Grants
NIGMS NIH HHS · GM26976 · United States
Databases
GENBANK
M34052
Corrections
ErratumIn
PMID: 2023956
External Links
PubMed source DOI: 10.1073/pnas.87.13.5227 PMC Full Text View journal details
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