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PMID: 23602809 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Review

Conformational flexibility and structural dynamics in GPCR-mediated G protein activation: a perspective.

Journal of molecular biology ·Vol. 425 ·No. 13 ·2013-07-10 ·Pages 2288-98

Preininger AM, Meiler J, Hamm HE

Abstract

Structure and dynamics of G proteins and their cognate receptors, both alone and in complex, are becoming increasingly accessible to experimental techniques. Understanding the conformational changes and timelines that govern these changes can lead to new insights into the processes of ligand binding and associated G protein activation. Experimental systems may involve the use of, or otherwise stabilize, non-native environments. This can complicate our understanding of structural and dynamic features of processes such as the ionic lock, tryptophan toggle, and G protein flexibility. While elements in the receptor's transmembrane helices and the C-terminal α5 helix of Gα undergo well-defined structural changes, regions subject to conformational flexibility may be important in fine-tuning the interactions between activated receptors and G proteins. The pairing of computational and experimental approaches will continue to provide powerful tools to probe the conformation and dynamics of receptor-mediated G protein activation.

MeSH Terms
Allosteric Regulation Crystallography, X-Ray GTP-Binding Proteins/chemistry,metabolism Models, Molecular Molecular Dynamics Simulation Protein Conformation Receptors, G-Protein-Coupled/chemistry,metabolism Signal Transduction
Chemicals
Receptors, G-Protein-Coupled GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Preininger Anita M
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232-6600, USA. Anita.Preininger@vanderbilt.edu
Meiler Jens
Hamm Heidi E
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Article Info
Journal
Journal of molecular biology
Abbr.
J Mol Biol
ISSN
1089-8638
Published
2013-07-10
Epub
2013-00-16
Pages
2288-98
Language
English
Region
England
NLM ID
2985088R
PMCID
PMC3686903
Subset
IM
Grants
NIGMS NIH HHS · R01 GM099842 · United States
NIGMS NIH HHS · GM095633 · United States
NIDDK NIH HHS · R01 DK097376 · United States
NIGMS NIH HHS · R01 GM080403 · United States
NIGMS NIH HHS · R01 GM095633 · United States
NEI NIH HHS · EY006062 · United States
NIMH NIH HHS · R01 MH090192 · United States
NIGMS NIH HHS · GM080403 · United States
NIGMS NIH HHS · GM099842 · United States
NEI NIH HHS · R01 EY006062 · United States
NIMH NIH HHS · MH090192 · United States
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