Home LiteratureArticle Details
PMID: 2351720 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization and description of "Campylobacter upsaliensis" isolated from human feces.

Journal of clinical microbiology ·Vol. 28 ·No. 5 ·1990-05-00 ·Pages 1039-46

Goossens H, Pot B, Vlaes L, Van den Borre C, Van den Abbeele R, Van Naelten C, Levy J, Cogniau H, Marbehant P, Verhoef J

Abstract

During a 3-year period, "Campylobacter upsaliensis" was isolated from 99 patients. Phenotypic characterization and numerical analysis of protein electrophoregrams showed evidence that "C. upsaliensis" is a distinct Campylobacter species with unique characteristics. The MBCs of 13 antibiotics were determined. In general, these organisms were highly susceptible to drugs that were present in the selective isolation media, making none of the available selective media suitable for the isolation of "C. upsaliensis." Ten strains were found to be resistant to erythromycin (MBCs, greater than or equal to 12.50 mg/liter). Plasmid DNA was detectable in 89 of the 99 strains; 16 plasmid profiles could be identified. Plasmid pattern 16, containing four plasmids of 52, 32, 5.5, and 2.6 megadaltons, represented 60.7% of the plasmid-containing strains. None of the "C. upsaliensis" strains could be agglutinated with antisera against heat-labile antigens from C. jejuni, C. coli, or C. laridis. "C. upsaliensis" was found to be susceptible to serum killing and was readily phagocytized by human polymorphonuclear cells.

MeSH Terms
Bacterial Proteins/isolation & purification Blood Bactericidal Activity Campylobacter/drug effects,genetics,isolation & purification Campylobacter Infections/microbiology Diarrhea/microbiology Drug Resistance, Microbial Electrophoresis, Polyacrylamide Gel Feces/microbiology Humans Phagocytosis Phenotype Plasmids Serotyping Species Specificity
Chemicals
Bacterial Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Goossens H
World Health Organization Collaborating Centre for Enteric Campylobacter, St. Pieters University Hospital, Brussels, Belgium.
Pot B
Vlaes L
Van den Borre C
Van den Abbeele R
Van Naelten C
Levy J
Cogniau H
Marbehant P
Verhoef J
References (30)
30 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. "Campylobacter upsaliensis" isolated from cats as identified by DNA relatedness and biochemical features.
    J Clin Microbiol. 1989 Oct;27(10):2376-8 PMID: 2584385
  3. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis.
    Infect Immun. 1981 Feb;31(2):775-82 PMID: 7216474
  4. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors.
    J Clin Microbiol. 1982 May;15(5):761-8 PMID: 7096555
  5. Blood-free selective medium for isolation of Campylobacter jejuni from feces.
    J Clin Microbiol. 1984 Feb;19(2):169-71 PMID: 6699146
  6. Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis.
    J Clin Microbiol. 1984 Mar;19(3):342-6 PMID: 6325487
  7. New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli, and "Campylobacter laridis".
    J Clin Microbiol. 1984 Oct;20(4):636-40 PMID: 6490850
  8. The use of membrane filters applied directly to the surface of agar plates for the isolation of Campylobacter jejuni from feces.
    Pathology. 1984 Jul;16(3):263-5 PMID: 6393011
  9. Comparative in vitro activities of aztreonam, ciprofloxacin, norfloxacin, ofloxacin, HR 810 (a new cephalosporin), RU28965 (a new macrolide), and other agents against enteropathogens.
    Antimicrob Agents Chemother. 1985 Mar;27(3):388-92 PMID: 3158276
  10. Urease-positive thermophilic campylobacters.
    Lancet. 1985 May 25;1(8439):1217-8 PMID: 2860418
  11. DNA relatedness and biochemical features of Campylobacter spp. isolated in central and South Australia.
    J Clin Microbiol. 1985 Jul;22(1):71-4 PMID: 2991331
  12. DNA homology studies of the catalase-negative campylobacters and "Campylobacter fecalis," an emended description of Campylobacter sputorum, and proposal of the neotype strain of Campylobacter sputorum.
    Can J Microbiol. 1985 Sep;31(9):823-31 PMID: 4084862
  13. Plasmid profiles of clinical isolates of Campylobacter with no or weak catalase activity.
    S Afr Med J. 1986 Sep 13;70(6):337-8 PMID: 3750139
  14. Characterization of Wolinella spp., Campylobacter concisus, Bacteroides gracilis, and Eikenella corrodens by polyacrylamide gel electrophoresis.
    J Clin Microbiol. 1986 Oct;24(4):562-5 PMID: 3533976
  15. Modified selective medium for isolation of Campylobacter spp. from feces: comparison with Preston medium, a blood-free medium, and a filtration system.
    J Clin Microbiol. 1986 Nov;24(5):840-3 PMID: 3771769
  16. Hydrolysis of indoxyl acetate by Campylobacter species.
    J Clin Microbiol. 1987 Aug;25(8):1560-1 PMID: 3624449
  17. Rapid and improved gas-liquid chromatography technique for detection of hippurate hydrolysis by Campylobacter jejuni and Campylobacter coli.
    J Clin Microbiol. 1987 Sep;25(9):1776-8 PMID: 3654950
  18. Electrophoretic protein typing of Campylobacter jejuni subspecies "doylei" (nitrate-negative campylobacter-like organisms) from human faeces and gastric mucosa.
    Eur J Epidemiol. 1988 Sep;4(3):277-83 PMID: 3053235
  19. Numerical analysis of electrophoretic protein patterns of Campylobacter laridis and allied thermophilic campylobacters from the natural environment.
    J Appl Bacteriol. 1988 Jul;65(1):69-78 PMID: 3209518
  20. Human disease associated with "Campylobacter upsaliensis" (catalase-negative or weakly positive Campylobacter species) in the United States.
    J Clin Microbiol. 1989 Jan;27(1):66-73 PMID: 2913038
  21. Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance.
    Clin Microbiol Rev. 1988 Apr;1(2):228-43 PMID: 2852997
  22. Characterization of Campylobacter cinaedi and C. fennelliae antigens and analysis of the human immune response.
    J Infect Dis. 1989 Apr;159(4):635-40 PMID: 2466910
  23. "Campylobacter upsaliensis" isolated from blood cultures of pediatric patients.
    J Clin Microbiol. 1989 Apr;27(4):657-9 PMID: 2723034
  24. Direct isolation of atypical thermophilic Campylobacter species from human feces on selective agar medium.
    J Clin Microbiol. 1989 Apr;27(4):668-70 PMID: 2723035
  25. Identification of 'Campylobacter upsaliensis' and other catalase-negative campylobacters from paediatric blood cultures by numerical analysis of electrophoretic protein patterns.
    FEMS Microbiol Lett. 1989 Apr;49(2-3):145-50 PMID: 2744416
  26. Semisolid blood-free selective-motility medium for the isolation of campylobacters from stool specimens.
    J Clin Microbiol. 1989 May;27(5):1077-80 PMID: 2745681
  27. Strain variation in Campylobacter pylori detected by numerical analysis of one-dimensional electrophoretic protein patterns.
    Antonie Van Leeuwenhoek. 1989 Mar;55(3):253-67 PMID: 2757368
  28. Identification of EF group 22 campylobacters from gastroenteritis cases as Campylobacter concisus.
    J Clin Microbiol. 1989 Aug;27(8):1775-81 PMID: 2768465
  29. Isolation and characterization of catalase-negative and catalase-weak strains of Campylobacter species, including "Campylobacter upsaliensis," from humans with gastroenteritis.
    J Clin Microbiol. 1989 Sep;27(9):2042-5 PMID: 2778067
  30. Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns.
    J Gen Microbiol. 1975 Apr;87(2):333-42 PMID: 1141858
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.28.5.1039-1046.1990
Published
1990-05-00
Pages
1039-46
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC267860
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.28.5.1039-1046.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2