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PMID: 2768465 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of EF group 22 campylobacters from gastroenteritis cases as Campylobacter concisus.

Journal of clinical microbiology ·Vol. 27 ·No. 8 ·1989-08-00 ·Pages 1775-81

Vandamme P, Falsen E, Pot B, Hoste B, Kersters K, De Ley J

Abstract

EF (E. Falsen) group 22, a group of Campylobacter strains sorted out by routine immunotyping among unidentified or misidentified human nonoral clinical specimens, was characterized by numerical analysis of gel electrophoretic protein profiles and immunotyping. The protein electrophoretic and immunotyping analyses, DNA:DNA hybridizations, and the DNA base composition demonstrated unambiguously that all EF group 22 strains belong to Campylobacter concisus. EF group 22 strains have DNA binding values of at least 42% with the type strain of C. concisus, showing a considerable degree of genomic heterogeneity. The isolation from blood, esophagus, stomach, duodenum, and feces of humans in association with different gastrointestinal disorders considerably extends the clinical significance of this species. Our results indicate that sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunotyping are excellent tools for the identification of the fastidious C. concisus strains and relatives.

MeSH Terms
Adult Animals Bacterial Proteins/analysis Base Composition Campylobacter/analysis,classification,genetics Campylobacter Infections/microbiology Child, Preschool Cross Reactions DNA, Bacterial/analysis,metabolism Electrophoresis, Polyacrylamide Gel Female Gastroenteritis/microbiology Humans Infant Male Middle Aged Nucleic Acid Hybridization Sequence Homology, Nucleic Acid Serotyping
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vandamme P
Laboratorium voor Microbiologie en Microbiële Genetica, Rijksuniversiteit Gent, Belgium.
Falsen E
Pot B
Hoste B
Kersters K
De Ley J
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.27.8.1775-1781.1989
Published
1989-08-00
Pages
1775-81
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC267670
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.27.8.1775-1781.1989 PMC Full Text View journal details
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