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PMID: 5438045 Published · ppublish English Journal Article

Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid.

Journal of bacteriology ·Vol. 101 ·No. 3 ·1970-03-00 ·Pages 738-54

De Ley J

Abstract

The equations currently used for the calculation of the chemical base composition of deoxyribonucleic acid (DNA), expressed as moles per cent guanine plus cytosine (% GC), from either buoyant density (rho) or midpoint of thermal denaturation (T(m)) were recalculated by using only sets of data on DNA determined with the same strains. All available information from the literature was screened and supplemented by unpublished data. The results were calculated by regression and correlation analysis and treated statistically. From the data on 96 strains of bacteria, it was calculated that% GC = 2.44 (T(m) - 69.4). T(m) appears to be unaffected by the substitution of cytosine by hydroxymethylcytosine. This equation is also valid for nonbacterial DNA. From the data on 84 strains of bacteria, the relation% GC = 1038.47 (-1.6616) was calculated. The constants in this equation are slightly modified when data on nonbacterial DNA are included. Both correlations differ only slightly from those currently used, but now they lean on a statistically sound basis. As a control, the relation between rho and T(m) was calculated from data of 197 strains; it agrees excellently with the above two equations.

MeSH Terms
Bacteria/analysis Chemical Phenomena Chemistry Cytosine/analysis DNA/analysis DNA, Bacterial/analysis DNA, Viral/analysis Eukaryota/analysis Guanine/analysis Mathematics Viruses/analysis Yeasts/analysis
Chemicals
DNA, Bacterial DNA, Viral Guanine Cytosine DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
De Ley J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.101.3.738-754.1970
Published
1970-03-00
Pages
738-54
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC250386
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.101.3.738-754.1970 PMC Full Text View journal details
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