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PMID: 2744416 Published · ppublish English Journal Article

Identification of 'Campylobacter upsaliensis' and other catalase-negative campylobacters from paediatric blood cultures by numerical analysis of electrophoretic protein patterns.

FEMS microbiology letters ·Vol. 49 ·No. 2-3 ·1989-04-00 ·Pages 145-50

Owen RJ, Morgan DD, Costas M, Lastovica A

Abstract

Twenty-one strains of catalase-negative campylobacters from paediatric blood cultures were characterized by one-dimensional SDS-PAGE of cellular proteins. A further 11 Campylobacter strains were included for reference purposes. The partial protein patterns were used as the basis of a numerical analysis, which showed that 17 hippurate-negative strains had a high similarity to 'C. upsaliensis' (r greater than or equal to 0.82) irrespective of their geographical location, and that three hippurate-positive isolates had a high similarity (r greater than or equal to 0.87) to C. jejuni subsp. jejuni. One hippurate-positive CNW strain was not identified. The analysis of SDS-PAGE protein patterns proved an excellent method of characterizing these thermophilic campylobacter as they were difficult to identify by traditional methods.

MeSH Terms
Bacterial Proteins/analysis Campylobacter/analysis,classification,isolation & purification Campylobacter Infections/blood,microbiology Catalase/analysis Child Electrophoresis, Polyacrylamide Gel Hippurates/analysis Humans Species Specificity
Chemicals
Bacterial Proteins Hippurates Catalase hippuric acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Owen R J
National Collection of Type Cultures, Central Public Health Laboratory, London, U.K.
Morgan D D
Costas M
Lastovica A
Article Info
Journal
FEMS microbiology letters
Abbr.
FEMS Microbiol Lett
ISSN
0378-1097
Published
1989-04-00
Pages
145-50
Language
English
Region
England
NLM ID
7705721
Subset
IM
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