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PMID: 23451120 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A cis-acting element present within the Gag open reading frame negatively impacts on the activity of the HIV-1 IRES.

PloS one ·Vol. 8 ·No. 2 ·2013-00-00 ·Pages e56962

Valiente-Echeverría F, Vallejos M, Monette A, Pino K, Letelier A, Huidobro-Toro JP, Mouland AJ, López-Lastra M

Abstract

Translation initiation from the human immunodeficiency virus type-1 (HIV-1) mRNA can occur through a cap or an IRES dependent mechanism. Cap-dependent translation initiation of the HIV-1 mRNA can be inhibited by the instability element (INS)-1, a cis-acting regulatory element present within the gag open reading frame (ORF). In this study we evaluated the impact of the INS-1 on HIV-1 IRES-mediated translation initiation. Using heterologous bicistronic mRNAs, we show that the INS-1 negatively impact on HIV-1 IRES-driven translation in in vitro and in cell-based experiments. Additionally, our results show that the inhibitory effect of the INS-1 is not general to all IRESes since it does not hinder translation driven by the HCV IRES. The inhibition by the INS-1 was partially rescued in cells by the overexpression of the viral Rev protein or hnRNPA1.

MeSH Terms
Genes, gag/genetics HIV-1/genetics HeLa Cells Humans Immunoblotting Open Reading Frames/genetics Regulatory Sequences, Nucleic Acid/genetics Reverse Transcriptase Polymerase Chain Reaction rev Gene Products, Human Immunodeficiency Virus/genetics
Chemicals
rev Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Valiente-Echeverría Fernando
Laboratorio de Virología Molecular, Instituto Milenio de Inmunología e Inmunoterapia, Centro de Investigaciones Médicas, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
Vallejos Maricarmen
Monette Anne
Pino Karla
Letelier Alejandro
Huidobro-Toro J Pablo
Mouland Andrew J
López-Lastra Marcelo
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0056962
Published
2013-00-00
Epub
2013-00-25
Pages
e56962
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3581557
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0056962 PMC Full Text View journal details
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