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PMID: 19737937 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human immunodeficiency virus type 1 (HIV-1) induces the cytoplasmic retention of heterogeneous nuclear ribonucleoprotein A1 by disrupting nuclear import: implications for HIV-1 gene expression.

The Journal of biological chemistry ·Vol. 284 ·No. 45 ·2009-11-06 ·Pages 31350-62

Monette A, Ajamian L, López-Lastra M, Mouland AJ

Abstract

Human immunodeficiency virus type 1 (HIV-1) co-opts host proteins and cellular machineries to its advantage at every step of the replication cycle. Here we show that HIV-1 enhances heterogeneous nuclear ribonucleoprotein (hnRNP) A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm. The latter was dependent on the nuclear export of the unspliced viral genomic RNA (vRNA) and to alterations in the abundance and localization of the FG-repeat nuclear pore glycoprotein p62. hnRNP A1 and vRNA remain colocalized in the cytoplasm supporting a post-nuclear function during the late stages of HIV-1 replication. Consistently, we show that hnRNP A1 acts as an internal ribosomal entry site trans-acting factor up-regulating internal ribosome entry site-mediated translation initiation of the HIV-1 vRNA. The up-regulation and cytoplasmic retention of hnRNP A1 by HIV-1 would ensure abundant expression of viral structural proteins in cells infected with HIV-1.

MeSH Terms
Active Transport, Cell Nucleus Cell Nucleus/genetics,metabolism Cytoplasm/genetics,metabolism Gene Expression Regulation, Viral HIV Infections/metabolism,virology HIV-1/genetics,physiology HeLa Cells Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B/genetics,physiology Humans Virus Internalization
Chemicals
Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B hnRNPA1 protein, human
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Monette Anne
HIV-1 RNA Trafficking Laboratory, Lady Davis Institute for Medical Research-Sir Mortimer B. Davis Jewish General Hospital, Montréal, Québec H3T 1E2, Canada.
Ajamian Lara
López-Lastra Marcelo
Mouland Andrew J
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M109.048736
Published
2009-11-06
Epub
2009-00-08
Pages
31350-62
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2781532
Subset
IM
Grants
Canadian Institutes of Health Research · Canada
External Links
PubMed source DOI: 10.1074/jbc.M109.048736 PMC Full Text View journal details
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