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PMID: 23142872 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.

Nature methods ·Vol. 9 ·No. 12 ·2012-12-00 ·Pages 1192-4

Martin L, Meier M, Lyons SM, Sit RV, Marzluff WF, Quake SR, Chang HY

Abstract

We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.

MeSH Terms
Animals Base Sequence Gene Library Glutathione Transferase Humans Microfluidics/methods Nucleotide Motifs Phylogeny RNA/chemistry RNA Probes
Chemicals
RNA Probes RNA Glutathione Transferase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Martin Lance
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.
Meier Matthias
Lyons Shawn M
Sit Rene V
Marzluff William F
Quake Stephen R
Chang Howard Y
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16 references, click to expand
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7105
DOI
10.1038/nmeth.2225
Published
2012-12-00
Epub
2012-00-11
Pages
1192-4
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC3863600
Subset
IM
Grants
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R01 GM029832 · United States
NHGRI NIH HHS · R01-HG004361 · United States
NIGMS NIH HHS · R01GM29832 · United States
NHGRI NIH HHS · R01 HG004361 · United States
External Links
PubMed source DOI: 10.1038/nmeth.2225 PMC Full Text View journal details
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