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PMID: 22251872 Published · epublish English Journal Article Research Support, Non-U.S. Gov't Review

Protein-RNA interactions: new genomic technologies and perspectives.

Nature reviews. Genetics ·Vol. 13 ·No. 2 ·2012-01-18 ·Pages 77-83

König J, Zarnack K, Luscombe NM, Ule J

Abstract

RNA-binding proteins are key players in the regulation of gene expression. In this Progress article, we discuss state-of-the-art technologies that can be used to study individual RNA-binding proteins or large complexes such as the ribosome. We also describe how these approaches can be used to study interactions with different types of RNAs, including nascent transcripts, mRNAs, microRNAs and ribosomal RNAs, in order to investigate transcription, RNA processing and translation. Finally, we highlight current challenges in data analysis and the future steps that are needed to obtain a quantitative and high-resolution picture of protein-RNA interactions on a genome-wide scale.

MeSH Terms
Animals Computational Biology Genomics/methods High-Throughput Nucleotide Sequencing Humans RNA/metabolism RNA-Binding Proteins/metabolism Ribonucleoproteins/metabolism
Chemicals
RNA-Binding Proteins Ribonucleoproteins RNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
König Julian
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK.
Zarnack Kathi
Luscombe Nicholas M
Ule Jernej
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Article Info
Journal
Nature reviews. Genetics
Abbr.
Nat Rev Genet
ISSN
1471-0064
DOI
10.1038/nrg3141
Published
2012-01-18
Epub
2012-00-18
Pages
77-83
Language
English
Region
England
NLM ID
100962779
Subset
IM
Grants
Wellcome Trust · 089701 · United Kingdom
European Research Council · 206726 · International
Medical Research Council · MC_U105185858 · United Kingdom
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1038/nrg3141 View journal details
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