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PMID: 2304473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The ubiquitous octamer-binding protein(s) is sufficient for transcription of immunoglobulin genes.

Molecular and cellular biology ·Vol. 10 ·No. 3 ·1990-03-00 ·Pages 982-90

Johnson DG, Carayannopoulos L, Capra JD, Tucker PW, Hanke JH

Abstract

All immunoglobulin genes contain a conserved octanucleotide promoter element, ATGCAAAT, which has been shown to be required for their normal B-cell-specific transcription. Proteins that bind this octamer have been purified, and cDNAs encoding octamer-binding proteins have been cloned. Some of these proteins (referred to as OTF-2) are lymphoid specific, whereas at least one other, and possibly more (referred to as OTF-1), is found ubiquitously in all cell types. The exact role of these different proteins in directing the tissue-specific expression of immunoglobulin genes is unclear. We have identified two human pre-B-cell lines that contain extremely low levels of OTF-2 yet still express high levels of steady-state immunoglobulin heavy-chain mRNA in vivo and efficiently transcribe an immunoglobulin gene in vitro. Addition of a highly enriched preparation of OTF-1 made from one of these pre-B cells or from HeLa cells specifically stimulated in vitro transcription of an immunoglobulin gene. Furthermore, OFT-1 appeared to have approximately the same transactivation ability as OTF-2 when normalized for binding activity. These results suggest that OTF-1, without OTF-2, is sufficient for transcription of immunoglobulin genes and that OTF-2 alone is not responsible for the B-cell-specific regulation of immunoglobulin gene expression.

MeSH Terms
Base Sequence Blotting, Northern Cell Nucleus/physiology DNA-Binding Proteins/physiology Gene Expression Regulation Genes, Immunoglobulin Humans In Vitro Techniques Lymphocytes/physiology Molecular Sequence Data Oligonucleotide Probes Promoter Regions, Genetic Restriction Mapping Transcription Factors/physiology Transcription, Genetic Tumor Cells, Cultured
Chemicals
DNA-Binding Proteins Oligonucleotide Probes Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Johnson D G
Department of Microbiology, Southwestern Medical Center, Dallas, Texas 75235-9096.
Carayannopoulos L
Capra J D
Tucker P W
Hanke J H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.3.982-990.1990
Published
1990-03-00
Pages
982-90
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360948
Subset
IM
Grants
NIAID NIH HHS · AI-180186 · United States
NIAID NIH HHS · AI-18499 · United States
NIGMS NIH HHS · GM-31689 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.3.982-990.1990 PMC Full Text View journal details
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