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PMID: 3387240 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A conserved heptamer upstream of the IgH promoter region octamer can be the site of a coordinate protein-DNA interaction.

Nucleic acids research ·Vol. 16 ·No. 12 ·1988-06-24 ·Pages 5503-14

Landolfi NF, Yin XM, Capra JD, Tucker PW

Abstract

Immunoglobulin genes contain a conserved eight base sequence element 5' to the site of transcription initiation. This octamer can serve as a site for the binding of nuclear proteins which are presumably involved in the cell type specific expression of this family of genes. In studying the binding of nuclear proteins to this conserved sequence element, we have detected a protein interaction that involves, in addition to the octamer, nucleotides which are immediately upstream. We have characterized this additional contact as a sequence specific interaction with a heptameric sequence element (CTCATGA) that is conserved among Ig heavy chain promoters. Protein binding to the heptamer is unique in that it is dependent upon the proximity and orientation of, as well as protein interaction with, the conserved octamer.

MeSH Terms

Base Sequence DNA/genetics Genes, Immunoglobulin Immunoglobulin Variable Region/genetics Molecular Sequence Data Nuclear Proteins/metabolism Promoter Regions, Genetic Protein Binding Transcription, Genetic

Chemicals

Immunoglobulin Variable Region Nuclear Proteins DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Landolfi N F

Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.

Yin X M

Capra J D

Tucker P W

References (23)

23 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.12.5503

Published

1988-06-24

Pages

5503-14

Language

English

Region

England

NLM ID

0411011

PMCID

PMC336781

Subset

Grants

NIAID NIH HHS · AI-18016 · United States

NIGMS NIH HHS · GM-1751 · United States

NIGMS NIH HHS · GM-31689 · United States

External Links

PubMed source DOI: 10.1093/nar/16.12.5503 PMC Full Text View journal details

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A conserved heptamer upstream of the IgH promoter region octamer can be the site of a coordinate protein-DNA interaction.

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