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PMID: 2296602 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Maize regulatory gene opaque-2 encodes a protein with a "leucine-zipper" motif that binds to zein DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 1 ·1990-01-00 ·Pages 46-50

Schmidt RJ, Burr FA, Aukerman MJ, Burr B

Abstract

The opaque-2 locus (o2) in maize regulates the expression of many members of the zein multigene family of storage proteins. cDNA clones for a wild-type allele of the (o2) locus (O2) were isolated from a maize endosperm cDNA library and sequenced. We found a 258-nucleotide 5' leader sequence containing three short open reading frames followed by a sequence specifying a protein of 437 amino acids. The presumptive amino acid sequence of the protein (O2) specified by the O2 cDNA contains a "leucine-zipper" domain characteristic of some mammalian and fungal transcription activation factors. lacZ-O2 fusion constructs, using nearly the entire coding region of O2 or only a fragment specifying the leucine-zipper domain, were expressed in Escherichia coli. In an in vitro binding assay, the beta-galactosidase-O2 fusion proteins bound to two specific regions on the 5' side of the coding sequence in a zein genomic clone. This suggests that the O2 protein affects zein transcription through direct interaction with one or more zein promoter elements.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular DNA/genetics,isolation & purification,metabolism DNA-Binding Proteins/genetics,isolation & purification,metabolism Genes Genes, Regulator Molecular Sequence Data Nucleic Acid Hybridization Plants/genetics Recombinant Fusion Proteins/isolation & purification,metabolism Restriction Mapping Sequence Homology, Nucleic Acid Zea mays/genetics Zein/genetics
Chemicals
DNA-Binding Proteins Recombinant Fusion Proteins DNA Zein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schmidt R J
Department of Biology, University of California, San Diego, La Jolla 92093.
Burr F A
Aukerman M J
Burr B
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.1.46
Published
1990-01-00
Pages
46-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53196
Subset
IM
Grants
NCI NIH HHS · CA9345 · United States
NIGMS NIH HHS · GM31093 · United States
NIGMS NIH HHS · GM41286 · United States
Databases
GENBANK
M29411
External Links
PubMed source DOI: 10.1073/pnas.87.1.46 PMC Full Text View journal details
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