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PMID: 22815985 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The dynamic changes of DNA methylation and histone modifications of salt responsive transcription factor genes in soybean.

PloS one ·Vol. 7 ·No. 7 ·2012-00-00 ·Pages e41274

Song Y, Ji D, Li S, Wang P, Li Q, Xiang F

Abstract

Epigenetic modification contributes to the regulation of gene expression and plant development under salinity stress. Here we describe the identification of 49 soybean transcription factors by microarray analysis as being inducible by salinity stress. A semi-quantitative RT-PCR-based expression assay confirmed the salinity stress inducibility of 45 of these 49 transcription factors, and showed that ten of them were up-regulated when seedlings were exposed to the demethylation agent 5-aza-2-deoxycytidine. Salinity stress was shown to affect the methylation status of four of these ten transcription factors (one MYB, one b-ZIP and two AP2/DREB family members) using a combination of bisulfite sequencing and DNA methylation-sensitive DNA gel blot analysis. ChIP analysis indicated that the activation of three of the four DNA methylated transcription factors was correlated with an increased level of histone H3K4 trimethylation and H3K9 acetylation, and/or a reduced level of H3K9 demethylation in various parts of the promoter or coding regions. Our results suggest a critical role for some transcription factors' activation/repression by DNA methylation and/or histone modifications in soybean tolerance to salinity stress.

MeSH Terms
Azacitidine/pharmacology Cytosine/chemistry DNA/chemistry,metabolism DNA Methylation Epigenesis, Genetic Gene Expression Regulation, Plant Histones/chemistry,metabolism Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic Salts/chemistry Sequence Analysis, DNA Soybeans/genetics,metabolism Thymine/chemistry Transcription Factors/metabolism Transcriptional Activation
Chemicals
Histones Salts Transcription Factors Cytosine DNA Azacitidine Thymine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Song Yuguang
The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Jinan, Shandong, China.
Ji Dandan
Li Shuo
Wang Peng
Li Qiang
Xiang Fengning
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0041274
Published
2012-00-00
Epub
2012-00-18
Pages
e41274
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3399865
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0041274 PMC Full Text View journal details
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