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PMID: 16326925 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ROR1/RPA2A, a putative replication protein A2, functions in epigenetic gene silencing and in regulation of meristem development in Arabidopsis.

The Plant cell ·Vol. 18 ·No. 1 ·2006-01-00 ·Pages 85-103

Xia R, Wang J, Liu C, Wang Y, Wang Y, Zhai J, Liu J, Hong X, Cao X, Zhu JK, Gong Z

Abstract

We screened for suppressors of repressor of silencing1 (ros1) using the silenced 35S promoter-neomycin phosphotransferase II (Pro(35S):NPTII) gene as a marker and identified two allelic mutants, ror1-1 and ror1-2 (for suppressor of ros1). Map-based cloning revealed that ROR1 encodes a 31-kD protein similar to DNA replication protein A2 (RPA2A). Mutations in ROR1 reactivate the silenced Pro(35S):NPTII gene but not RD29A promoter-luciferase in the ros1 mutant. DNA methylation in rDNA, centromeric DNA, and RD29A promoter regions is not affected by ror1. However, chromatin immunoprecipitation data suggest that histone H3 acetylation is increased and histone H3K9 dimethylation is decreased in the 35S promoter in the ror1 ros1 mutant compared with ros1. These results indicate that release of silenced Pro(35S):NPTII by ror1 mutations is independent of DNA methylation. ROR1/RPA2A is strongly expressed in shoot and root meristems. Mutations in ROR1/RPA2A affect cell division in meristems but not final cell sizes. Our work suggests important roles of ROR1/RPA2A in epigenetic gene silencing and in the regulation of plant development.

MeSH Terms
Amino Acid Sequence Arabidopsis/anatomy & histology,genetics,growth & development Arabidopsis Proteins/genetics,metabolism DNA Methylation DNA-Binding Proteins/genetics,metabolism Epigenesis, Genetic Gene Expression Regulation, Plant Gene Silencing Genes, Reporter Genetic Complementation Test Humans Meristem/cytology,growth & development Molecular Sequence Data Mutation Nuclear Proteins/genetics,metabolism Plants, Genetically Modified Promoter Regions, Genetic Recombinant Fusion Proteins/genetics,metabolism Sequence Alignment Transgenes Two-Hybrid System Techniques
Chemicals
Arabidopsis Proteins DNA-Binding Proteins Nuclear Proteins ROR1 protein, Arabidopsis ROS1 protein, Arabidopsis Recombinant Fusion Proteins
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Xia Ran
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing.
Wang Junguo
Liu Chunyan
Wang Yu
Wang Youqun
Zhai Jixian
Liu Jun
Hong Xuhui
Cao Xiaofeng
Zhu Jian-Kang
Gong Zhizhong
References (82)
82 references, click to expand
  1. A gateway cloning vector set for high-throughput functional analysis of genes in planta.
    Plant Physiol. 2003 Oct;133(2):462-9 PMID: 14555774
  2. The small RNA world.
    J Cell Sci. 2003 Dec 1;116(Pt 23):4689-93 PMID: 14600255
  3. Hsk1-Dfp1 is required for heterochromatin-mediated cohesion at centromeres.
    Nat Cell Biol. 2003 Dec;5(12):1111-6 PMID: 14625560
  4. Terminal flower2, an Arabidopsis homolog of heterochromatin protein1, counteracts the activation of flowering locus T by constans in the vascular tissues of leaves to regulate flowering time.
    Plant Cell. 2003 Dec;15(12):2856-65 PMID: 14630968
  5. Vernalization in Arabidopsis thaliana is mediated by the PHD finger protein VIN3.
    Nature. 2004 Jan 8;427(6970):159-64 PMID: 14712276
  6. Vernalization requires epigenetic silencing of FLC by histone methylation.
    Nature. 2004 Jan 8;427(6970):164-7 PMID: 14712277
  7. MGOUN3, an Arabidopsis gene with TetratricoPeptide-Repeat-related motifs, regulates meristem cellular organization.
    J Exp Bot. 2004 Mar;55(397):673-84 PMID: 14966212
  8. Arabidopsis histone deacetylase HDA6 is required for maintenance of transcriptional gene silencing and determines nuclear organization of rDNA repeats.
    Plant Cell. 2004 Apr;16(4):1021-34 PMID: 15037732
  9. BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis.
    Genes Dev. 2004 Apr 1;18(7):782-93 PMID: 15082530
  10. Involvement of putative SNF2 chromatin remodeling protein DRD1 in RNA-directed DNA methylation.
    Curr Biol. 2004 May 4;14(9):801-5 PMID: 15120073
  11. A novel Arabidopsis gene TONSOKU is required for proper cell arrangement in root and shoot apical meristems.
    Plant J. 2004 May;38(4):673-84 PMID: 15125773
  12. Genetic and functional diversification of small RNA pathways in plants.
    PLoS Biol. 2004 May;2(5):E104 PMID: 15024409
  13. DNA and histone methylation in plants.
    Trends Genet. 2004 Jun;20(6):244-51 PMID: 15145577
  14. Role of Arabidopsis ARGONAUTE4 in RNA-directed DNA methylation triggered by inverted repeats.
    Curr Biol. 2004 Jul 13;14(13):1214-20 PMID: 15242620
  15. Replication protein A phosphorylation and the cellular response to DNA damage.
    DNA Repair (Amst). 2004 Aug-Sep;3(8-9):1015-24 PMID: 15279788
  16. RNA-directed DNA methylation.
    J Cell Sci. 2004 Oct 1;117(Pt 21):4881-8 PMID: 15456843
  17. Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3.
    EMBO J. 2004 Oct 27;23(21):4286-96 PMID: 15457214
  18. Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase.
    Genes Dev. 1991 Sep;5(9):1589-600 PMID: 1885001
  19. The VERNALIZATION 2 gene mediates the epigenetic regulation of vernalization in Arabidopsis.
    Cell. 2001 Nov 16;107(4):525-35 PMID: 11719192
  20. Genome-wide profiling of DNA methylation reveals transposon targets of CHROMOMETHYLASE3.
    Curr Biol. 2002 Jan 8;12(1):65-8 PMID: 11790305
  21. Epigenetic codes for heterochromatin formation and silencing: rounding up the usual suspects.
    Cell. 2002 Feb 22;108(4):489-500 PMID: 11909520
  22. Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe.
    Nucleic Acids Res. 2002 Apr 1;30(7):1465-82 PMID: 11917007
  23. Control of CpNpG DNA methylation by the KRYPTONITE histone H3 methyltransferase.
    Nature. 2002 Apr 4;416(6880):556-60 PMID: 11898023
  24. Signaling in and out: control of cell division and differentiation in the shoot and root.
    Plant Cell. 2002;14 Suppl:S265-76 PMID: 12045282
  25. Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing.
    Curr Biol. 2002 Jul 9;12(13):1138-44 PMID: 12121623
  26. Interplay between two epigenetic marks. DNA methylation and histone H3 lysine 9 methylation.
    Curr Biol. 2002 Aug 20;12(16):1360-7 PMID: 12194816
  27. Dependence of heterochromatic histone H3 methylation patterns on the Arabidopsis gene DDM1.
    Science. 2002 Sep 13;297(5588):1871-3 PMID: 12077425
  28. Severe developmental defects, hypersensitivity to DNA-damaging agents, and lengthened telomeres in Arabidopsis MRE11 mutants.
    Plant Cell. 2002 Oct;14(10):2451-62 PMID: 12368497
  29. Depletion of MOM1 in non-dividing cells of Arabidopsis plants releases transcriptional gene silencing.
    EMBO Rep. 2002 Oct;3(10):951-5 PMID: 12231508
  30. Two regulatory levels of transcriptional gene silencing in Arabidopsis.
    Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13659-62 PMID: 12370435
  31. Chromatin dynamics in plants.
    Curr Opin Plant Biol. 2002 Dec;5(6):560-7 PMID: 12393020
  32. RNA-directed DNA methylation in Arabidopsis.
    Proc Natl Acad Sci U S A. 2002 Dec 10;99 Suppl 4:16499-506 PMID: 12169664
  33. HDA6, a putative histone deacetylase needed to enhance DNA methylation induced by double-stranded RNA.
    EMBO J. 2002 Dec 16;21(24):6832-41 PMID: 12486004
  34. An Arabidopsis SET domain protein required for maintenance but not establishment of DNA methylation.
    EMBO J. 2002 Dec 16;21(24):6842-52 PMID: 12486005
  35. Initiation of DNA replication in multicellular eukaryotes.
    J Struct Biol. 2002 Oct-Dec;140(1-3):17-30 PMID: 12490150
  36. Arabidopsis thaliana DNA methylation mutants.
    Science. 1993 Jun 25;260(5116):1926-8 PMID: 8316832
  37. Cellular organisation of the Arabidopsis thaliana root.
    Development. 1993 Sep;119(1):71-84 PMID: 8275865
  38. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress.
    Plant Cell. 1994 Feb;6(2):251-64 PMID: 8148648
  39. Mutations in the gene encoding the 34 kDa subunit of yeast replication protein A cause defective S phase progression.
    J Mol Biol. 1995 Dec 8;254(4):595-607 PMID: 7500336
  40. The origin recognition complex, SIR1, and the S phase requirement for silencing.
    Science. 1997 Jun 6;276(5318):1547-51 PMID: 9171055
  41. Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism.
    Annu Rev Biochem. 1997;66:61-92 PMID: 9242902
  42. Association of the origin recognition complex with heterochromatin and HP1 in higher eukaryotes.
    Cell. 1997 Oct 31;91(3):311-23 PMID: 9363940
  43. Separable functions of ORC5 in replication initiation and silencing in Saccharomyces cerevisiae.
    Genetics. 1997 Nov;147(3):1053-62 PMID: 9383052
  44. The TERMINAL FLOWER2 (TFL2) gene controls the reproductive transition and meristem identity in Arabidopsis thaliana.
    Genetics. 1998 Jun;149(2):597-605 PMID: 9611176
  45. Distinct cytoplasmic and nuclear fractions of Drosophila heterochromatin protein 1: their phosphorylation levels and associations with origin recognition complex proteins.
    J Cell Biol. 1998 Jul 27;142(2):307-18 PMID: 9679132
  46. Role of WUSCHEL in regulating stem cell fate in the Arabidopsis shoot meristem.
    Cell. 1998 Dec 11;95(6):805-15 PMID: 9865698
  47. Maintenance of genomic methylation requires a SWI2/SNF2-like protein.
    Nat Genet. 1999 May;22(1):94-7 PMID: 10319870
  48. RNAi-mediated pathways in the nucleus.
    Nat Rev Genet. 2005 Jan;6(1):24-35 PMID: 15630419
  49. Role of chromatin modification in flowering-time control.
    Trends Plant Sci. 2005 Jan;10(1):30-5 PMID: 15642521
  50. The Arabidopsis HOMOLOGY-DEPENDENT GENE SILENCING1 gene codes for an S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing.
    Plant Cell. 2005 Feb;17(2):404-17 PMID: 15659630
  51. DNA methylation and epigenetics.
    Annu Rev Plant Biol. 2004;55:41-68 PMID: 15725056
  52. Plant nuclear RNA polymerase IV mediates siRNA and DNA methylation-dependent heterochromatin formation.
    Cell. 2005 Mar 11;120(5):613-22 PMID: 15766525
  53. RNA polymerase IV directs silencing of endogenous DNA.
    Science. 2005 Apr 1;308(5718):118-20 PMID: 15692015
  54. Transitions in distinct histone H3 methylation patterns at the heterochromatin domain boundaries.
    Science. 2001 Aug 10;293(5532):1150-5 PMID: 11498594
  55. ROS1, a repressor of transcriptional gene silencing in Arabidopsis, encodes a DNA glycosylase/lyase.
    Cell. 2002 Dec 13;111(6):803-14 PMID: 12526807
  56. Gardening the genome: DNA methylation in Arabidopsis thaliana.
    Nat Rev Genet. 2005 May;6(5):351-60 PMID: 15861207
  57. Pivotal role of AtSUVH2 in heterochromatic histone methylation and gene silencing in Arabidopsis.
    EMBO J. 2005 Apr 6;24(7):1418-29 PMID: 15775980
  58. Two types of replication protein A in seed plants.
    FEBS J. 2005 Jul;272(13):3270-81 PMID: 15978034
  59. Atypical RNA polymerase subunits required for RNA-directed DNA methylation.
    Nat Genet. 2005 Jul;37(7):761-5 PMID: 15924141
  60. A simple, rapid and quantitative method for preparing Arabidopsis protein extracts for immunoblot analysis.
    Plant J. 1999 Oct;20(2):251-7 PMID: 10571885
  61. Ectopic hypermethylation of flower-specific genes in Arabidopsis.
    Curr Biol. 2000 Feb 24;10(4):179-86 PMID: 10704409
  62. Disruption of the plant gene MOM releases transcriptional silencing of methylated genes.
    Nature. 2000 May 11;405(6783):203-6 PMID: 10821279
  63. A chromodomain protein, Swi6, performs imprinting functions in fission yeast during mitosis and meiosis.
    Cell. 2000 Apr 28;101(3):307-17 PMID: 10847685
  64. Endogenous targets of transcriptional gene silencing in Arabidopsis.
    Plant Cell. 2000 Jul;12(7):1165-78 PMID: 10899982
  65. DNA methylation and chromatin structure affect transcriptional and post-transcriptional transgene silencing in Arabidopsis.
    Curr Biol. 2000 Dec 14-28;10(24):1591-4 PMID: 11137011
  66. FASCIATA genes for chromatin assembly factor-1 in arabidopsis maintain the cellular organization of apical meristems.
    Cell. 2001 Jan 12;104(1):131-42 PMID: 11163246
  67. Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
    Nature. 2001 Mar 1;410(6824):116-20 PMID: 11242053
  68. Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
    Nature. 2001 Mar 1;410(6824):120-4 PMID: 11242054
  69. A role for DNA polymerase alpha in epigenetic control of transcriptional silencing in fission yeast.
    EMBO J. 2001 Jun 1;20(11):2857-66 PMID: 11387218
  70. Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation.
    Science. 2001 Jun 15;292(5524):2077-80 PMID: 11349138
  71. Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene.
    Genes Dev. 2001 Jul 15;15(14):1753-8 PMID: 11459824
  72. Two types of replication protein A 70 kDa subunit in rice, Oryza sativa: molecular cloning, characterization, and cellular & tissue distribution.
    Gene. 2001 Jul 11;272(1-2):335-43 PMID: 11470540
  73. Translating the histone code.
    Science. 2001 Aug 10;293(5532):1074-80 PMID: 11498575
  74. ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation.
    Science. 2003 Jan 31;299(5607):716-9 PMID: 12522258
  75. Cell division and morphological changes in the shoot apex of Arabidopsis thaliana during floral transition.
    Ann Bot. 2003 Apr;91(5):571-6 PMID: 12646501
  76. Plant meristems: a merry-go-round of signals.
    Curr Biol. 2003 Apr 29;13(9):R368-74 PMID: 12725756
  77. Mutations in Arabidopsis condensin genes disrupt embryogenesis, meristem organization and segregation of homologous chromosomes during meiosis.
    Development. 2003 Jul;130(14):3283-95 PMID: 12783798
  78. Arabidopsis TERMINAL FLOWER 2 gene encodes a heterochromatin protein 1 homolog and represses both FLOWERING LOCUS T to regulate flowering time and several floral homeotic genes.
    Plant Cell Physiol. 2003 Jun;44(6):555-64 PMID: 12826620
  79. EARLY BOLTING IN SHORT DAYS is related to chromatin remodeling factors and regulates flowering in Arabidopsis by repressing FT.
    Plant Cell. 2003 Jul;15(7):1552-62 PMID: 12837946
  80. PIE1, an ISWI family gene, is required for FLC activation and floral repression in Arabidopsis.
    Plant Cell. 2003 Jul;15(7):1671-82 PMID: 12837955
  81. Shoot apical meristem maintenance: the art of a dynamic balance.
    Trends Plant Sci. 2003 Aug;8(8):394-401 PMID: 12927973
  82. Plant-specific regulation of replication protein A2 (OsRPA2) from rice during the cell cycle and in response to ultraviolet light exposure.
    Planta. 2003 Jul;217(3):457-65 PMID: 14520573
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.037507
Published
2006-01-00
Epub
2005-00-02
Pages
85-103
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1323486
Subset
IM
Databases
GENBANK
DQ284987
External Links
PubMed source DOI: 10.1105/tpc.105.037507 PMC Full Text View journal details
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