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PMID: 12368497 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Severe developmental defects, hypersensitivity to DNA-damaging agents, and lengthened telomeres in Arabidopsis MRE11 mutants.

The Plant cell ·Vol. 14 ·No. 10 ·2002-10-00 ·Pages 2451-62

Bundock P, Hooykaas P

Abstract

The Mre11 protein is essential for the long-term genetic stability of the cell and acts to ensure the efficient repair of DNA damage. Vertebrate cells lacking Mre11 function are not viable. However, we report here that this is not the case in the model plant Arabidopsis. We have isolated two different Arabidopsis lines containing a T-DNA copy integrated at a different point in the MRE11 gene (AtMRE11). Both mutant plant lines were hypersensitive to DNA-damaging treatments but exhibited strikingly different developmental phenotypes. Furthermore, we also observed lengthened telomeres in these plant lines, showing that AtMre11 is involved in telomere maintenance. Thus, the lines we have isolated are unique tools with which to study in detail the role of AtMre11 in the mature plant.

MeSH Terms
Amino Acid Sequence Arabidopsis/drug effects,genetics,growth & development Arabidopsis Proteins/genetics,metabolism DNA Damage/drug effects,radiation effects DNA Repair/genetics,physiology Gene Expression Regulation, Plant/drug effects,radiation effects Methyl Methanesulfonate/pharmacology Molecular Sequence Data Mutation Sequence Homology, Amino Acid Telomere/drug effects,genetics X-Rays
Chemicals
Arabidopsis Proteins Methyl Methanesulfonate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bundock Paul
Institute for Molecular Plant Sciences, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. bundock@rulbim.leidenuniv.nl
Hooykaas Paul
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.005959
Email
bundock@rulbim.leidenuniv.nl
Published
2002-10-00
Pages
2451-62
Language
English
Region
England
NLM ID
9208688
PMCID
PMC151228
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.005959 PMC Full Text View journal details
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