Home LiteratureArticle Details
PMID: 12140324 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Increased telomere length and hypersensitivity to DNA damaging agents in an Arabidopsis KU70 mutant.

Nucleic acids research ·Vol. 30 ·No. 15 ·2002-08-01 ·Pages 3395-400

Bundock P, van Attikum H, Hooykaas P

Abstract

We have identified a putative homologue of the KU70 gene (AtKU70) from Arabidopsis thaliana. In order to study its function in plants we have isolated an A.thaliana line that contains a T-DNA inserted into AtKU70. Plants homozygous for this insertion appear normal and are fertile. In other organisms the KU70 gene has been shown to play a role in the repair of DNA damage induced by ionising radiation (IR) and by radiomimetic chemicals such as methylmethane sulfonate (MMS). We show that AtKU70(-/-) plants are hypersensitive to IR and MMS, and thus the AtKU70 gene plays a similar role in DNA repair in plants as in other organisms. The KU70 gene also plays a role in maintaining telomere length. Yeast and mammalian cells deficient for Ku70 have shortened telomeres. When we studied the telomeres in the AtKU70(-/-) plants we found unexpectedly that they were significantly longer (>30 kb) than was found in wild-type plants (2-4 kb). We propose several hypotheses to explain this telomere lengthening in the AtKU70(-/-) plants.

MeSH Terms
Amino Acid Sequence Antigens, Nuclear Arabidopsis/drug effects,radiation effects,ultrastructure Arabidopsis Proteins/genetics,physiology DNA Damage DNA Helicases DNA, Plant/ultrastructure DNA-Binding Proteins/genetics,physiology Genes, Plant Ku Autoantigen Methyl Methanesulfonate/pharmacology Molecular Sequence Data Mutation Nuclear Proteins/genetics Radiation Tolerance Saccharomyces cerevisiae Proteins Sequence Alignment Telomere/ultrastructure
Chemicals
Antigens, Nuclear Arabidopsis Proteins DNA, Plant DNA-Binding Proteins KU70 protein, Arabidopsis Nuclear Proteins Saccharomyces cerevisiae Proteins high affinity DNA-binding factor, S cerevisiae Methyl Methanesulfonate DNA Helicases XRCC5 protein, human Xrcc6 protein, human Ku Autoantigen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bundock Paul
Institute of Molecular Plant Sciences, Sectie 2A, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. bundock@rulbim.leidenuniv.nl
van Attikum Haico
Hooykaas Paul
References (34)
34 references, click to expand
  1. T-DNA as an insertional mutagen in Arabidopsis.
    Plant Cell. 1999 Dec;11(12):2283-90 PMID: 10590158
  2. Mre11 and Ku70 interact in somatic cells, but are differentially expressed in early meiosis.
    Nat Genet. 1999 Oct;23(2):194-8 PMID: 10508516
  3. Control of human telomere length by TRF1 and TRF2.
    Mol Cell Biol. 2000 Mar;20(5):1659-68 PMID: 10669743
  4. Recombination in telomere-length maintenance.
    Trends Biochem Sci. 2000 Apr;25(4):200-4 PMID: 10754555
  5. Ku recruits the XRCC4-ligase IV complex to DNA ends.
    Mol Cell Biol. 2000 May;20(9):2996-3003 PMID: 10757784
  6. Recombination: a frank view of exchanges and vice versa.
    Curr Opin Cell Biol. 2000 Jun;12(3):286-92 PMID: 10801454
  7. Interaction of human Ku70 with TRF2.
    FEBS Lett. 2000 Sep 8;481(1):81-5 PMID: 10984620
  8. Ku acts in a unique way at the mammalian telomere to prevent end joining.
    Genes Dev. 2000 Nov 15;14(22):2807-12 PMID: 11090128
  9. The function of a stem-loop in telomerase RNA is linked to the DNA repair protein Ku.
    Nat Genet. 2001 Jan;27(1):64-7 PMID: 11138000
  10. Disruption of the Arabidopsis RAD50 gene leads to plant sterility and MMS sensitivity.
    Plant J. 2001 Jan;25(1):31-41 PMID: 11169180
  11. RAD50 function is essential for telomere maintenance in Arabidopsis.
    Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1711-6 PMID: 11172016
  12. Living with genome instability: plant responses to telomere dysfunction.
    Science. 2001 Mar 2;291(5509):1797-800 PMID: 11230697
  13. Mammalian Ku86 protein prevents telomeric fusions independently of the length of TTAGGG repeats and the G-strand overhang.
    EMBO Rep. 2000 Sep;1(3):244-52 PMID: 11256607
  14. A plant gene encoding a Myb-like protein that binds telomeric GGTTTAG repeats in vitro.
    J Biol Chem. 2001 May 11;276(19):16511-9 PMID: 11278537
  15. The absence of the dna-dependent protein kinase catalytic subunit in mice results in anaphase bridges and in increased telomeric fusions with normal telomere length and G-strand overhang.
    Mol Cell Biol. 2001 Jun;21(11):3642-51 PMID: 11340158
  16. Identification of bacterial homologues of the Ku DNA repair proteins.
    FEBS Lett. 2001 Jul 6;500(3):186-8 PMID: 11445083
  17. Effects of DNA nonhomologous end-joining factors on telomere length and chromosomal stability in mammalian cells.
    Curr Biol. 2001 Aug 7;11(15):1192-6 PMID: 11516951
  18. Nej1p, a cell type-specific regulator of nonhomologous end joining in yeast.
    Curr Biol. 2001 Oct 16;11(20):1611-7 PMID: 11676923
  19. DNA-PKcs is critical for telomere capping.
    Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15084-8 PMID: 11742099
  20. An alternative pathway for yeast telomere maintenance rescues est1- senescence.
    Cell. 1993 Apr 23;73(2):347-60 PMID: 8477448
  21. Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae.
    Mol Cell Biol. 1996 Aug;16(8):4189-98 PMID: 8754818
  22. Requirement for Ku80 in growth and immunoglobulin V(D)J recombination.
    Nature. 1996 Aug 8;382(6591):551-5 PMID: 8700231
  23. Control of telomere length by the human telomeric protein TRF1.
    Nature. 1997 Feb 20;385(6618):740-3 PMID: 9034193
  24. Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity, and inability to support V(D)J recombination.
    Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8076-81 PMID: 9223317
  25. Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination In vivo.
    J Exp Med. 1997 Sep 15;186(6):921-9 PMID: 9294146
  26. Growth retardation and leaky SCID phenotype of Ku70-deficient mice.
    Immunity. 1997 Nov;7(5):653-65 PMID: 9390689
  27. Hypersensitivity of Ku80-deficient cell lines and mice to DNA damage: the effects of ionizing radiation on growth, survival, and development.
    Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13588-93 PMID: 9391070
  28. Telomeres and double-strand breaks: trying to make ends meet.
    Trends Cell Biol. 1998 Sep;8(9):339-42 PMID: 9728393
  29. Developmental control of telomere lengths and telomerase activity in plants.
    Plant Cell. 1998 Oct;10(10):1691-8 PMID: 9761795
  30. Involvement of Arabidopsis thaliana ribosomal protein S27 in mRNA degradation triggered by genotoxic stress.
    EMBO J. 1999 Jan 15;18(2):490-9 PMID: 9889204
  31. Catalytic subunit of DNA-dependent protein kinase: impact on lymphocyte development and tumorigenesis.
    Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1403-8 PMID: 9990036
  32. The repressor which binds the -75 GATA motif of the GPB promoter contains Ku70 as the DNA binding subunit.
    Nucleic Acids Res. 1999 Apr 1;27(7):1656-63 PMID: 10075997
  33. Ku, a DNA repair protein with multiple cellular functions?
    Mutat Res. 1999 May 14;434(1):3-15 PMID: 10377944
  34. Disruption of the telomerase catalytic subunit gene from Arabidopsis inactivates telomerase and leads to a slow loss of telomeric DNA.
    Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14813-8 PMID: 10611295
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkf445
Email
bundock@rulbim.leidenuniv.nl
Published
2002-08-01
Pages
3395-400
Language
English
Region
England
NLM ID
0411011
PMCID
PMC137071
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkf445 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2