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PMID: 9391070 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Hypersensitivity of Ku80-deficient cell lines and mice to DNA damage: the effects of ionizing radiation on growth, survival, and development.

Nussenzweig A, Sokol K, Burgman P, Li L, Li GC

Abstract

We recently have shown that mice deficient for the 86-kDa component (Ku80) of the DNA-dependent protein kinase exhibit growth retardation and a profound deficiency in V(D)J (variable, diversity, and joining) recombination. These defects may be related to abnormalities in DNA metabolism that arise from the inability of Ku80 mutant cells to process DNA double-strand breaks. To further characterize the role of Ku80 in DNA double-strand break repair, we have generated embryonic stem cells and pre-B cells and examined their response to ionizing radiation. Ku80(-/-) embryonic stem cells are more sensitive than controls to gamma-irradiation, and pre-B cells derived from Ku80 mutant mice display enhanced spontaneous and gamma-ray-induced apoptosis. We then determined the effects of ionizing radiation on the survival, growth, and lymphocyte development in Ku80-deficient mice. Ku80(-/-) mice display a hypersensitivity to gamma-irradiation, characterized by loss of hair pigmentation, severe injury to the gastrointestinal tract, and enhanced mortality. Exposure of newborn Ku80(-/-) mice to sublethal doses of ionizing radiation enhances their growth retardation and results in the induction of T cell-specific differentiation. However, unlike severe combined immunodeficient mice, radiation-induced T cell development in Ku80(-/-) mice is not accompanied by extensive thymocyte proliferation. The response of Ku80-deficient cell lines and mice to DNA-damaging agents provides important insights into the role of Ku80 in growth regulation, lymphocyte development, and DNA repair.

MeSH Terms
Animals Antigens, Nuclear B-Lymphocytes/cytology,immunology,radiation effects Cell Differentiation/radiation effects Cell Line DNA Damage DNA Helicases DNA Repair DNA-Binding Proteins/genetics,metabolism Female Gamma Rays/adverse effects Growth/genetics,radiation effects Growth Disorders/etiology,genetics Intestines/pathology,radiation effects Ku Autoantigen Male Mice Mice, Knockout Mice, SCID Nuclear Proteins/genetics,metabolism Radiation Tolerance T-Lymphocytes/cytology,immunology,radiation effects
Chemicals
Antigens, Nuclear DNA-Binding Proteins Nuclear Proteins DNA Helicases XRCC5 protein, human Xrcc6 protein, human Xrcc6 protein, mouse Ku Autoantigen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nussenzweig A
Departments of Medical Physics and Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Sokol K
Burgman P
Li L
Li G C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1997-12-09
Pages
13588-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC28350
Subset
IM
Grants
NCI NIH HHS · T32 CA061801 · United States
NCI NIH HHS · CA-61801 · United States
NCI NIH HHS · R01 CA056909 · United States
NCI NIH HHS · CA-56909 · United States
NCI NIH HHS · CA-31397 · United States
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