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PMID: 11486038 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mre11 complex and DNA replication: linkage to E2F and sites of DNA synthesis.

Molecular and cellular biology ·Vol. 21 ·No. 17 ·2001-09-00 ·Pages 6006-16

Maser RS, Mirzoeva OK, Wells J, Olivares H, Williams BR, Zinkel RA, Farnham PJ, Petrini JH

Abstract

We show that the Mre11 complex associates with E2F family members via the Nbs1 N terminus. This association and Nbs1 phosphorylation are correlated with S-phase checkpoint proficiency, whereas neither is sufficient individually for checkpoint activation. The Nbs1 E2F interaction occurred near the Epstein-Barr virus origin of replication as well as near a chromosomal replication origin in the c-myc promoter region and was restricted to S-phase cells. The Mre11 complex colocalized with PCNA at replication forks throughout S phase, both prior to and coincident with the appearance of nascent DNA. These data suggest that the Mre11 complex suppresses genomic instability through its influence on both the regulation and progression of DNA replication.

MeSH Terms
Animals Binding Sites Cell Cycle Proteins Cell Line DNA Repair Enzymes DNA Replication DNA-Binding Proteins/metabolism E2F Transcription Factors HeLa Cells Humans MRE11 Homologue Protein Mice Nuclear Proteins/metabolism Phosphorylation S Phase Signal Transduction Transcription Factors/metabolism Tumor Cells, Cultured
Chemicals
Cell Cycle Proteins DNA-Binding Proteins E2F Transcription Factors MRE11 protein, human Mre11a protein, mouse Nuclear Proteins Transcription Factors MRE11 Homologue Protein DNA Repair Enzymes
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Maser R S
Laboratory of Genetics, University of Wisconsin Medical School, 445 Henry Mall, Madison, WI 53706, USA.
Mirzoeva O K
Wells J
Olivares H
Williams B R
Zinkel R A
Farnham P J
Petrini J H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.17.6006-6016.2001
Published
2001-09-00
Pages
6006-16
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87318
Subset
IM
Grants
NIGMS NIH HHS · R37 GM059413 · United States
NIGMS NIH HHS · GM59413 · United States
NCI NIH HHS · CA45240 · United States
NIGMS NIH HHS · R01 GM059413 · United States
NCI NIH HHS · CA09681 · United States
NCI NIH HHS · T32 CA009681 · United States
NCI NIH HHS · R01 CA045240 · United States
External Links
PubMed source DOI: 10.1128/MCB.21.17.6006-6016.2001 PMC Full Text View journal details
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