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PMID: 12231508 Published · ppublish English Journal Article

Depletion of MOM1 in non-dividing cells of Arabidopsis plants releases transcriptional gene silencing.

EMBO reports ·Vol. 3 ·No. 10 ·2002-10-00 ·Pages 951-5

Tariq M, Habu Y, Paszkowski J

Abstract

Mitotic and meiotic inheritance of epigenetic information is coupled to the reproduction of chromatin conformation and DNA methylation patterns. This implies that the S phase of the cell cycle provides a window of opportunity for changes in epigenetic determination. Recent studies, however, have suggested that chromatin structure is also rather dynamic in quiescent cells of multicellular eukaryotes and that silent heterochromatic regions can become accessible to transcription. Such epigenetic flexibility in differentiated tissues could be of physiological importance. The mechanisms and molecular components involved are of great interest but as yet unknown. We examined MOM1 (Morpheus' Molecule 1), a regulator of transcriptional gene silencing (TGS) that acts independently of DNA methylation, for its role in the maintenance of TGS in non-dividing, differentiated cells. The results provide evidence that TGS maintenance mediated by MOM1 is a dynamic process that can be modified in non-dividing cells of mature plant organs by depletion of MOM1.

MeSH Terms
ATPases Associated with Diverse Cellular Activities Animals Arabidopsis/genetics,physiology Arabidopsis Proteins Blotting, Northern Cell Cycle DNA Methylation Gene Silencing Genetic Vectors Immunohistochemistry Introns Nuclear Proteins/biosynthesis Phenotype Plant Proteins/biosynthesis Plants, Genetically Modified Promoter Regions, Genetic Reverse Transcriptase Polymerase Chain Reaction S Phase Time Factors Transcription Factors/biosynthesis Transcription, Genetic
Chemicals
Arabidopsis Proteins Nuclear Proteins Plant Proteins Transcription Factors ATPases Associated with Diverse Cellular Activities MOM protein, Arabidopsis
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tariq Muhammad
Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland. tariq@fmi.ch
Habu Yoshiki
Paszkowski Jerzy
References (31)
31 references, click to expand
  1. Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa-DPa transcription factor.
    EMBO J. 2002 Mar 15;21(6):1360-8 PMID: 11889041
  2. Heterochromatin: new possibilities for the inheritance of structure.
    Curr Opin Genet Dev. 2002 Apr;12(2):178-87 PMID: 11893491
  3. The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly.
    Mol Cell. 2002 Jun;9(6):1191-200 PMID: 12086617
  4. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  5. Role of DNA replication in the repression of silent mating type loci in yeast.
    Nature. 1984 Nov 15-21;312(5991):247-51 PMID: 6390211
  6. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
    EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
  7. The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicing.
    Cell. 1989 Aug 11;58(3):473-83 PMID: 2758463
  8. Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway.
    Plant J. 1996 Jul;10(1):71-82 PMID: 8758979
  9. Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues.
    Plant J. 1996 Jul;10(1):107-21 PMID: 8758981
  10. Reduced DNA methylation in Arabidopsis thaliana results in abnormal plant development.
    Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8449-54 PMID: 8710891
  11. Demethylation-induced developmental pleiotropy in Arabidopsis.
    Science. 1996 Aug 2;273(5275):654-7 PMID: 8662558
  12. Characterization of proteins that interact with the GTP-bound form of the regulatory GTPase Ran in Arabidopsis.
    Plant J. 1997 Jan;11(1):93-103 PMID: 9025305
  13. DNA methylation directs a time-dependent repression of transcription initiation.
    Curr Biol. 1997 Mar 1;7(3):157-65 PMID: 9395433
  14. Concurrent replication and methylation at mammalian origins of replication.
    Mol Cell Biol. 1998 Jun;18(6):3475-82 PMID: 9584187
  15. Chromosome methylation patterns during mammalian preimplantation development.
    Genes Dev. 1998 Jul 15;12(14):2108-13 PMID: 9679055
  16. Functional analysis of regulatory sequences controlling PR-1 gene expression in Arabidopsis.
    Plant J. 1998 Oct;16(2):223-33 PMID: 9839467
  17. Expression of CKS1At in Arabidopsis thaliana indicates a role for the protein in both the mitotic and the endoreduplication cycle.
    Planta. 1999 Feb;207(4):496-504 PMID: 10093894
  18. Coactivator and corepressor complexes in nuclear receptor function.
    Curr Opin Genet Dev. 1999 Apr;9(2):140-7 PMID: 10322133
  19. Cell cycling and cell enlargement in developing leaves of Arabidopsis.
    Dev Biol. 1999 Nov 15;215(2):407-19 PMID: 10545247
  20. Yeast heterochromatin is a dynamic structure that requires silencers continuously.
    Genes Dev. 2000 Feb 15;14(4):452-63 PMID: 10691737
  21. Disruption of the plant gene MOM releases transcriptional silencing of methylated genes.
    Nature. 2000 May 11;405(6783):203-6 PMID: 10821279
  22. Endogenous targets of transcriptional gene silencing in Arabidopsis.
    Plant Cell. 2000 Jul;12(7):1165-78 PMID: 10899982
  23. Endoreduplication in higher plants.
    Plant Mol Biol. 2000 Aug;43(5-6):735-45 PMID: 11089873
  24. The transcriptome of Arabidopsis thaliana during systemic acquired resistance.
    Nat Genet. 2000 Dec;26(4):403-10 PMID: 11101835
  25. DNA methylation and chromatin structure affect transcriptional and post-transcriptional transgene silencing in Arabidopsis.
    Curr Biol. 2000 Dec 14-28;10(24):1591-4 PMID: 11137011
  26. Modulation of a transcription factor counteracts heterochromatic gene silencing in Drosophila.
    Cell. 2001 Mar 23;104(6):839-47 PMID: 11290322
  27. Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation.
    Science. 2001 Jun 15;292(5524):2077-80 PMID: 11349138
  28. Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis.
    Plant Cell. 2001 Jul;13(7):1653-68 PMID: 11449057
  29. Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene.
    Genes Dev. 2001 Jul 15;15(14):1753-8 PMID: 11459824
  30. Expression and stability of Arabidopsis CDC6 are associated with endoreplication.
    Plant Cell. 2001 Dec;13(12):2671-86 PMID: 11752380
  31. Identification of a small molecule inhibitor of Sir2p.
    Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15113-8 PMID: 11752457
Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1093/embo-reports/kvf195
Email
tariq@fmi.ch
Published
2002-10-00
Epub
2002-00-13
Pages
951-5
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1307620
Subset
IM
External Links
PubMed source DOI: 10.1093/embo-reports/kvf195 PMC Full Text View journal details
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