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PMID: 15082530 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis.

Genes & development ·Vol. 18 ·No. 7 ·2004-04-01 ·Pages 782-93

Takeda S, Tadele Z, Hofmann I, Probst AV, Angelis KJ, Kaya H, Araki T, Mengiste T, Mittelsten Scheid O, Shibahara K, Scheel D, Paszkowski J

Abstract

DNA repair associated with DNA replication is important for the conservation of genomic sequence information, whereas reconstitution of chromatin after replication sustains epigenetic information. We have isolated and characterized mutations in the BRU1 gene of Arabidopsis that suggest a novel link between these underlying maintenance mechanisms. Bru1 plants are highly sensitive to genotoxic stress and show stochastic release of transcriptional gene silencing. They also show increased intrachromosomal homologous recombination and constitutively activated expression of poly (ADP-ribose) polymerase-2 (AtPARP-2), the induction of which is associated with elevated DNA damage. Bru1 mutations affect the stability of heterochromatin organization but do not interfere with genome-wide DNA methylation. BRU1 encodes a novel nuclear protein with two predicted protein-protein interaction domains. The developmental abnormalities characteristic of bru1 mutant plants resemble those triggered by mutations in genes encoding subunits of chromatin assembly factor (CAF-1), the condensin complex, or MRE11. Comparison of bru1 with these mutants indicates cooperative roles in the replication and stabilization of chromatin structure, providing a novel link between chromatin replication, epigenetic inheritance, S-phase DNA damage checkpoints, and the regulation of meristem development.

MeSH Terms
Arabidopsis/genetics,growth & development Chromatin Assembly Factor-1 Chromosomal Proteins, Non-Histone/genetics,metabolism Cloning, Molecular Comet Assay DNA Damage DNA Methylation DNA Replication DNA, Plant/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Endodeoxyribonucleases/genetics,metabolism Epigenesis, Genetic/genetics Exodeoxyribonucleases/genetics,metabolism Gene Expression Regulation, Plant Gene Silencing Genes, Plant/physiology Heterochromatin/chemistry,genetics,metabolism In Situ Hybridization, Fluorescence Meristem/growth & development Mutation/genetics Plants, Genetically Modified Protein Interaction Mapping RNA, Messenger/genetics,metabolism Recombination, Genetic Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
Chromatin Assembly Factor-1 Chromosomal Proteins, Non-Histone DNA, Plant DNA-Binding Proteins Heterochromatin RNA, Messenger Saccharomyces cerevisiae Proteins Endodeoxyribonucleases Exodeoxyribonucleases MRE11 protein, S cerevisiae
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Takeda Shin
Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland. shin.takeda@bioveg.unige.ch
Tadele Zerihun
Hofmann Ingo
Probst Aline V
Angelis Karel J
Kaya Hidetaka
Araki Takashi
Mengiste Tesfaye
Mittelsten Scheid Ortrun
Shibahara Kei-ichi
Scheel Dierk
Paszkowski Jerzy
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.295404
Email
shin.takeda@bioveg.unige.ch
Published
2004-04-01
Pages
782-93
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC387418
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.295404 PMC Full Text View journal details
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