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PMID: 18365246 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis.

Planta ·Vol. 228 ·No. 2 ·2008-07-00 ·Pages 225-40

Liao Y, Zou HF, Wei W, Hao YJ, Tian AG, Huang J, Liu YF, Zhang JS, Chen SY

Abstract

From soybean plant, 131 bZIP genes were identified and named as GmbZIPs. The GmbZIPs can be classified into ten groups and more than one third of these GmbZIPs are responsive to at least one of the four treatments including ABA, salt, drought and cold stresses. Previous studies have shown that group A bZIP proteins are involved in ABA and stress signaling. We now chose four non-group A genes to study their features. The four proteins GmbZIP44, GmbZIP46, GmbZIP62 and GmbZIP78 belong to the group S, I, C and G, respectively, and can bind to GLM (GTGAGTCAT), ABRE (CCACGTGG) and PB-like (TGAAAA) elements with differential affinity in both the yeast one-hybrid assay and in vitro gel-shift analysis. GmbZIP46 can form homodimer or heterodimer with GmbZIP62 or GmMYB76. Transgenic Arabidopsis plants overexpressing the GmbZIP44, GmbZIP62 or GmbZIP78 showed reduced ABA sensitivity. However, all the transgenic plants were more tolerant to salt and freezing stresses when compared with the Col plants. The GmbZIP44, GmbZIP62 and GmbZIP78 may function in ABA signaling through upregulation of ABI1 and ABI2 and play roles in stress tolerance through regulation of various stress-responsive genes. These results indicate that GmbZIP44, GmbZIP62 and GmbZIP78 are negative regulators of ABA signaling and function in salt and freezing tolerance.

MeSH Terms
Abscisic Acid/metabolism Arabidopsis/genetics,physiology Basic-Leucine Zipper Transcription Factors/genetics Cloning, Molecular Cold Temperature Dimerization Gene Expression Multigene Family Phylogeny Plants, Genetically Modified/genetics,physiology Salinity Signal Transduction Soybeans/genetics Substrate Specificity Transcriptional Activation Water/metabolism
Chemicals
Basic-Leucine Zipper Transcription Factors Water Abscisic Acid
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Liao Yong
Plant Gene Research Center, National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101 Beijing, China.
Zou Hong-Feng
Wei Wei
Hao Yu-Jun
Tian Ai-Guo
Huang Jian
Liu Yun-Feng
Zhang Jin-Song
Chen Shou-Yi
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-008-0731-3
Published
2008-07-00
Epub
2008-00-26
Pages
225-40
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-008-0731-3 View journal details
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