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PMID: 16001083 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Distinct regulation of histone H3 methylation at lysines 27 and 9 by CpG methylation in Arabidopsis.

The EMBO journal ·Vol. 24 ·No. 15 ·2005-08-03 ·Pages 2783-91

Mathieu O, Probst AV, Paszkowski J

Abstract

Transcriptional activity and structure of chromatin are correlated with patterns of covalent DNA and histone modification. Previous studies have revealed that high levels of histone H3 dimethylation at lysine 9 (H3K9me2), characteristic of transcriptionally silent heterochromatin in Arabidopsis, require hypermethylation of DNA at CpG sites. Here, we report that CpG hypermethylation characteristic of heterochromatin specifically prevented H3K27 trimethylation (H3K27me3). H3K27 mono- and dimethylation mark silent heterochromatin independently of DNA methylation. Upon loss of CpG methylation, there was target-specific enrichment of H3K27me3 in heterochromatin that correlated with transcriptional reactivation. Moreover, using the kyp mutant affected in H3K9me2, we showed that changes in H3K27me3 occurred independently of the levels of H3K9me2. Therefore, CpG methylation provides distinct and direct information for a specific subset of histone methylation marks. The observed independence of the regulation of H3K9 and H3K27 methylation by CpG methylation refines the recently proposed combinatorial histone code involving these two marks.

MeSH Terms
Arabidopsis/genetics,metabolism Biomarkers CpG Islands/genetics DNA Methylation DNA, Ribosomal/metabolism Histones/metabolism Immunohistochemistry Lysine/metabolism RNA, Ribosomal, 5S/metabolism Transcription, Genetic
Chemicals
Biomarkers DNA, Ribosomal Histones RNA, Ribosomal, 5S Lysine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mathieu Olivier
Laboratory of Plant Genetics, University of Geneva, Geneva, Switzerland. oliver.mathieu@bioveg.unige.ch
Probst Aline V
Paszkowski Jerzy
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600743
Email
oliver.mathieu@bioveg.unige.ch
Published
2005-08-03
Epub
2005-00-07
Pages
2783-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1182238
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600743 PMC Full Text View journal details
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