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PMID: 2251283 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression and chromosomal localization of a lymphocyte K+ channel gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 23 ·1990-12-00 ·Pages 9411-5

Grissmer S, Dethlefs B, Wasmuth JJ, Goldin AL, Gutman GA, Cahalan MD, Chandy KG

Abstract

We recently isolated a family of three closely related mouse K+ channel genes (MK1, MK2, and MK3) with coding regions contained in single uninterrupted exons. Here we have used patch-clamp recordings from Xenopus oocytes injected with mRNA to show that MK3 encodes a channel with biophysical and pharmacological properties indistinguishable from those of voltage-gated type n K+ channels in T cells. In addition, we used the polymerase chain reaction to demonstrate the presence of MK3 mRNA in T cells. These data suggest that MK3 may encode the T-cell voltage-gated type n K+ channel. We also show that MK3 and MK2 are localized on human chromosomes 13 and 12, respectively.

Related Genes
MK1 MK2 MK3
MeSH Terms
Animals Base Sequence Chromosome Mapping Electrophysiology/methods Female Membrane Potentials Molecular Sequence Data Multigene Family Oligonucleotide Probes Oocytes/physiology Potassium Channels/genetics,physiology Rats T-Lymphocytes/physiology Xenopus
Chemicals
Oligonucleotide Probes Potassium Channels
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Grissmer S
Department of Physiology and Biophysics, University of California, Irvine 92715.
Dethlefs B
Wasmuth J J
Goldin A L
Gutman G A
Cahalan M D
Chandy K G
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.23.9411
Published
1990-12-00
Pages
9411-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55175
Subset
IM
Grants
NIAID NIH HHS · AI21366 · United States
NIAID NIH HHS · AI24783 · United States
NINDS NIH HHS · NS26729 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.23.9411 PMC Full Text View journal details
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