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PMID: 2247061 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6374-80

Wevrick R, Earnshaw WC, Howard-Peebles PN, Willard HF

Abstract

A familial, constitutionally rearranged human chromosome 17 is deleted for much of the DNA in its centromeric region but retains full mitotic centromere activity. Fluorescence in situ hybridization, pulsed-field gel electrophoresis, and Southern blot analysis of the residual centromeric region revealed a approximately 700-kb centromeric array of tandemly repeated alpha satellite DNA that was only approximately 20 to 30% as large as a normal array. This deletion was associated with a reduction in the amount of the centromere-specific antigen CENP-B detected by indirect immunofluorescence. The coincidence of the primary constriction, the small residual array of alpha satellite DNA, and the reduced amount of detectable CENP-B support the hypothesis that CENP-B is associated with alpha satellite DNA. Furthermore, the finding that both the deleted chromosome 17 and its derivative supernumerary fragment retained mitotic function and possess centromeric protein antigens suggests that human centromeres are structurally and functionally repetitive.

MeSH Terms
Autoantigens/genetics Cell Line Centromere Protein B Chromosomal Proteins, Non-Histone/genetics Chromosome Deletion Chromosome Mapping Chromosomes, Human, Pair 17 DNA, Satellite/genetics DNA-Binding Proteins Gene Rearrangement Humans Metaphase Nucleic Acid Hybridization Restriction Mapping
Chemicals
Autoantigens CENPB protein, human Centromere Protein B Chromosomal Proteins, Non-Histone DNA, Satellite DNA-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wevrick R
Department of Molecular and School of Medicine, Medical Genetics, University of Toronto, Ontario, Canada.
Earnshaw W C
Howard-Peebles P N
Willard H F
References (53)
53 references, click to expand
  1. Autoantibody to centromere (kinetochore) in scleroderma sera.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1627-31 PMID: 6966403
  2. Chromosome walking shows a highly homologous repetitive sequence present in all the centromere regions of fission yeast.
    EMBO J. 1986 May;5(5):1011-21 PMID: 15957216
  3. Kinetochore structure, duplication, and distribution in mammalian cells: analysis by human autoantibodies from scleroderma patients.
    J Cell Biol. 1981 Oct;91(1):95-102 PMID: 7298727
  4. The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber.
    Int Rev Cytol. 1982;79:1-58 PMID: 6185450
  5. A sensitive and dependable assay for distinguishing hamster and human X-linked steroid sulfatase activity in somatic cell hybrids.
    Hum Genet. 1984;66(2-3):272-5 PMID: 6585346
  6. Compound kinetochores of the Indian muntjac. Evolution by linear fusion of unit kinetochores.
    Chromosoma. 1984;91(1):1-11 PMID: 6525895
  7. Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma.
    Chromosoma. 1985;91(3-4):313-21 PMID: 2579778
  8. Chromatin structure of altered yeast centromeres.
    Proc Natl Acad Sci U S A. 1988 Jan;85(1):175-9 PMID: 2829168
  9. Structural organization and functional analysis of centromeric DNA in the fission yeast Schizosaccharomyces pombe.
    Mol Cell Biol. 1988 Feb;8(2):754-63 PMID: 2832735
  10. Construction of functional artificial minichromosomes in the fission yeast Schizosaccharomyces pombe.
    Proc Natl Acad Sci U S A. 1989 Jan;86(2):577-81 PMID: 2643117
  11. Alphoid satellite DNA is tightly associated with centromere antigens in human chromosomes throughout the cell cycle.
    Exp Cell Res. 1989 Mar;181(1):181-96 PMID: 2917601
  12. Flow cytometry measurements of human chromosome kinetochore labeling.
    Cytometry. 1989 Mar;10(2):134-42 PMID: 2714105
  13. Anti-kinetochore antibodies: use as probes for inactive centromeres.
    Am J Hum Genet. 1985 Mar;37(2):425-30 PMID: 3885726
  14. Chromosome-specific organization of human alpha satellite DNA.
    Am J Hum Genet. 1985 May;37(3):524-32 PMID: 2988334
  15. Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome.
    Chromosoma. 1985;92(4):290-6 PMID: 2994966
  16. A cloned sequence, p82H, of the alphoid repeated DNA family found at the centromeres of all human chromosomes.
    Chromosoma. 1985;92(5):369-77 PMID: 2996845
  17. The mitotic stability of deletion derivatives of chromosome III in yeast.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):414-8 PMID: 3510434
  18. Chromosome-specific subfamilies within human alphoid repetitive DNA.
    J Mol Biol. 1986 Jan 20;187(2):185-96 PMID: 3009826
  19. Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2934-8 PMID: 3458254
  20. Chromosome length controls mitotic chromosome segregation in yeast.
    Cell. 1986 May 23;45(4):529-36 PMID: 3518944
  21. Indirect immunofluorescence of inactive centromeres as indicator of centromeric function.
    Hum Genet. 1986 May;73(1):12-6 PMID: 3519430
  22. Structure, organization, and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome.
    Mol Cell Biol. 1986 Sep;6(9):3156-65 PMID: 3785225
  23. Separation of large DNA molecules by contour-clamped homogeneous electric fields.
    Science. 1986 Dec 19;234(4783):1582-5 PMID: 3538420
  24. Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen.
    J Cell Biol. 1987 Apr;104(4):817-29 PMID: 2435739
  25. Genetic manipulation of centromere function.
    Mol Cell Biol. 1987 Jul;7(7):2397-405 PMID: 3302676
  26. Tubulin interaction with kinetochore proteins: analysis by in vitro assembly and chemical cross-linking.
    J Cell Biol. 1987 Aug;105(2):855-62 PMID: 3305522
  27. Structure of the major block of alphoid satellite DNA on the human Y chromosome.
    J Mol Biol. 1987 Jun 5;195(3):457-70 PMID: 2821279
  28. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.
    Nucleic Acids Res. 1987 Oct 12;15(19):7865-76 PMID: 2959907
  29. Organization and genomic distribution of "82H" alpha satellite DNA. Evidence for a low-copy or single-copy alphoid domain located on human chromosome 14.
    Hum Genet. 1988 Jan;78(1):27-32 PMID: 2828220
  30. Mitosis.
    Adv Cell Biol. 1971;2:225-97 PMID: 5005502
  31. A case of centric fission in man.
    Humangenetik. 1975;26(3):257-9 PMID: 1132882
  32. A possible function of constitutive heterochromatin: the bodyguard hypothesis.
    Genetics. 1975 Jun;79 Suppl:137-50 PMID: 1150080
  33. The possibility of latent centromeres and a proposed nomenclature system for total chromosome and whole arm translocations.
    Cytogenet Cell Genet. 1975;15(1):41-9 PMID: 1102265
  34. Assembly of microtubules onto kinetochores of isolated mitotic chromosomes of HeLa cells.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):4023-7 PMID: 1060085
  35. Centric fission of chromosome no. 4 in the mother of two patients with trisomy 4p.
    Hum Genet. 1976 Jan 28;31(1):121-5 PMID: 1248819
  36. Electron-microscopic study of the spindle and chromosome movement in the yeast Saccharomyces cerevisiae.
    J Cell Sci. 1976 Nov;22(2):219-42 PMID: 794073
  37. Chromosomal localization of complex and simple repeated human DNAs.
    Chromosoma. 1978 Mar 22;66(1):23-32 PMID: 639625
  38. Telomere replication, kinetochore organizers, and satellite DNA evolution.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4566-70 PMID: 291989
  39. Composite motifs and repeat symmetry in S. pombe centromeres: direct analysis by integration of NotI restriction sites.
    Cell. 1989 Jun 2;57(5):739-51 PMID: 2541922
  40. Determination of the breakpoints of 1;7 translocations in myelodysplastic syndrome by in situ hybridization using chromosome-specific alpha satellite DNA from human chromosomes 1 and 7.
    Cytogenet Cell Genet. 1989;50(1):49-53 PMID: 2743817
  41. Visualization of centromere proteins CENP-B and CENP-C on a stable dicentric chromosome in cytological spreads.
    Chromosoma. 1989 Jun;98(1):1-12 PMID: 2475307
  42. A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromeric satellite.
    J Cell Biol. 1989 Nov;109(5):1963-73 PMID: 2808515
  43. Long-range organization of tandem arrays of alpha satellite DNA at the centromeres of human chromosomes: high-frequency array-length polymorphism and meiotic stability.
    Proc Natl Acad Sci U S A. 1989 Dec;86(23):9394-8 PMID: 2594775
  44. A map of the centromere (primary constriction) in vertebrate chromosomes at metaphase.
    Prog Clin Biol Res. 1989;318:33-42 PMID: 2483271
  45. Human centromere structure: organization and potential role of alpha satellite DNA.
    Prog Clin Biol Res. 1989;318:9-18 PMID: 2696978
  46. Proteins of the inner and outer centromere of mitotic chromosomes.
    Genome. 1989;31(2):541-52 PMID: 2698830
  47. Functional analysis of a centromere from fission yeast: a role for centromere-specific repeated DNA sequences.
    Mol Cell Biol. 1990 May;10(5):1863-72 PMID: 2325639
  48. CENP-B: a major human centromere protein located beneath the kinetochore.
    J Cell Biol. 1990 May;110(5):1475-88 PMID: 2335558
  49. Structure of the human centromere at metaphase.
    Trends Biochem Sci. 1990 May;15(5):181-5 PMID: 2193435
  50. Y chromosome DNA haplotyping suggests that most European and Asian men are descended from one of two males.
    Genomics. 1990 Jul;7(3):325-30 PMID: 1973137
  51. Centromeres of budding and fission yeasts.
    Trends Genet. 1990 May;6(5):150-4 PMID: 2195725
  52. Pulsed-field gel analysis of alpha-satellite DNA at the human X chromosome centromere: high-frequency polymorphisms and array size estimate.
    Genomics. 1990 Aug;7(4):607-13 PMID: 1974881
  53. Balanced transmission of centromeric fission products in man.
    Hum Genet. 1980;54(1):127-8 PMID: 7390475
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6374-6380.1990
Published
1990-12-00
Pages
6374-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362913
Subset
IM
Grants
NIGMS NIH HHS · GM35212 · United States
NHGRI NIH HHS · HG00107 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.12.6374-6380.1990 PMC Full Text View journal details
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