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PMID: 6585346 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A sensitive and dependable assay for distinguishing hamster and human X-linked steroid sulfatase activity in somatic cell hybrids.

Human genetics ·Vol. 66 ·No. 2-3 ·1984-00-00 ·Pages 272-5

Willard HF, Holmes MT

Abstract

A radioisotopic assay is described to distinguish between Chinese hamster and human steroid sulfatase activity in extracts prepared from hamster X human somatic cell hybrids. This assay is based on different pH optima and provides a sensitive and unambiguous biochemical marker for the short arm of the human X chromosome and as well as for otherwise genetically inactivated X chromosomes in rodent X human hybrids.

MeSH Terms
Animals Chromosome Mapping Cricetinae Female Fibroblasts/enzymology Humans Hybrid Cells/enzymology Hydrogen-Ion Concentration Male Methods Mice Skin/cytology Species Specificity Steryl-Sulfatase Sulfatases/analysis,genetics X Chromosome
Chemicals
Sulfatases Steryl-Sulfatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Willard H F
Holmes M T
References (17)
17 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00286615
Published
1984-00-00
Pages
272-5
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00286615 View journal details
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