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PMID: 22194457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional analysis of the N-acetylglucosamine metabolic genes of Streptomyces coelicolor and role in control of development and antibiotic production.

Journal of bacteriology ·Vol. 194 ·No. 5 ·2012-03-00 ·Pages 1136-44

Świątek MA, Tenconi E, Rigali S, van Wezel GP

Abstract

N-acetylglucosamine, the monomer of chitin, is a favored carbon and nitrogen source for streptomycetes. Its intracellular catabolism requires the combined actions of the N-acetylglucosamine-6-phosphate (GlcNAc-6P) deacetylase NagA and the glucosamine-6-phosphate (GlcN-6P) deaminase/isomerase NagB. GlcNAc acts as a signaling molecule in the DasR-mediated nutrient sensing system, activating development and antibiotic production under poor growth conditions (famine) and blocking these processes under rich conditions (feast). In order to understand how a single nutrient can deliver opposite information according to the nutritional context, we carried out a mutational analysis of the nag metabolic genes nagA, nagB, and nagK. Here we show that the nag genes are part of the DasR regulon in Streptomyces coelicolor, which explains their transcriptional induction by GlcNAc. Most likely as the result of the intracellular accumulation of GlcN-6P, nagB deletion mutants fail to grow in the presence of GlcNAc. This toxicity can be alleviated by the additional deletion of nagA. We recently showed that in S. coelicolor, GlcNAc is internalized as GlcNAc-6P via the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). Considering the relevance of GlcNAc for the control of antibiotic production, improved insight into GlcNAc metabolism in Streptomyces may provide new leads toward biotechnological applications.

MeSH Terms
Acetylglucosamine/metabolism Anti-Bacterial Agents/biosynthesis Bacterial Proteins/genetics,metabolism DNA Mutational Analysis Gene Deletion Gene Expression Regulation, Bacterial Metabolic Networks and Pathways/genetics Regulon Streptomyces coelicolor/genetics,metabolism
Chemicals
Anti-Bacterial Agents Bacterial Proteins Acetylglucosamine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Świątek Magdalena A
Molecular Biotechnology, Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Tenconi Elodie
Rigali Sébastien
van Wezel Gilles P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
1098-5530
DOI
10.1128/JB.06370-11
Published
2012-03-00
Epub
2011-00-22
Pages
1136-44
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC3294797
Subset
IM
Corrections
CommentIn
PMID: 22892576
External Links
PubMed source DOI: 10.1128/JB.06370-11 PMC Full Text View journal details
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