Cloning, characterization and expression of beta-N-acetylglucosaminidase gene from Streptomyces thermoviolaceus OPC-520(1).

Biochimica et biophysica acta ·Vol. 1425 ·No. 2 ·1998-10-23 ·Pages 437-40

Tsujibo H, Hatano N, Mikami T, Izumizawa Y, Miyamoto K, Inamori Y

Abstract

The nagB gene encoding beta-N-acetylglucosaminidase from S. thermoviolaceus OPC-520 was cloned and sequenced. The nagB gene could encode a protein of 541 amino acids with a calculated molecular mass of 58274. NagB revealed significant similarities to beta-N-acetylhexosaminidases and chitobiases from bacteria, which are classified into family 20 glycosyl hydrolases. NagB effectively hydrolyzed all of the chitin oligosaccharides from dimer to hexamer.

MeSH Terms

Acetylglucosaminidase/biosynthesis,genetics Amino Acid Sequence Base Sequence Chitin/metabolism Cloning, Molecular Gene Expression Regulation, Enzymologic Molecular Sequence Data Sequence Alignment Streptomyces/enzymology,genetics

Chemicals

Chitin Acetylglucosaminidase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Tsujibo H

Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. tsujibo@oysun01.oups.ac.jp

Hatano N

Mikami T

Izumizawa Y

Miyamoto K

Inamori Y

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/s0304-4165(98)00098-1

tsujibo@oysun01.oups.ac.jp

Published

1998-10-23

Pages

437-40

Language

English

Region

Netherlands

NLM ID

0217513

Subset

Databases

GENBANK

AB015350

External Links

PubMed source DOI: 10.1016/s0304-4165(98)00098-1

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Cloning, characterization and expression of beta-N-acetylglucosaminidase gene from Streptomyces thermoviolaceus OPC-520(1).

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