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PMID: 16473843 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces.

Nucleic acids research ·Vol. 34 ·No. 3 ·2006-02-09 ·Pages e20

Khodakaramian G, Lissenden S, Gust B, Moir L, Hoskisson PA, Chater KF, Smith MC

Abstract

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phiC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.

MeSH Terms
Bacteriophages/genetics Gene Deletion Genes, Bacterial Genetic Markers Integrases/metabolism Recombination, Genetic Streptomyces coelicolor/genetics,virology Viral Proteins/metabolism
Chemicals
Genetic Markers Viral Proteins Cre recombinase Integrases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Khodakaramian Gholam
Institute of Genetics, University of Nottingham Nottingham NG7 2UH, UK.
Lissenden Sarah
Gust Bertolt
Moir Laura
Hoskisson Paul A
Chater Keith F
Smith Margaret C M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gnj019
Published
2006-02-09
Epub
2006-00-09
Pages
e20
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1363781
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BBS/B/11400 · United Kingdom
External Links
PubMed source DOI: 10.1093/nar/gnj019 PMC Full Text View journal details
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