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PMID: 21909073 Published · epublish English Journal Article Research Support, N.I.H., Extramural

Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1.

The EMBO journal ·Vol. 30 ·No. 22 ·2011-09-09 ·Pages 4652-64

Althoff T, Mills DJ, Popot JL, Kühlbrandt W

Abstract

The respiratory chain in the inner mitochondrial membrane contains three large multi-enzyme complexes that together establish the proton gradient for ATP synthesis, and assemble into a supercomplex. A 19-Å 3D map of the 1.7-MDa amphipol-solubilized supercomplex I(1)III(2)IV(1) from bovine heart obtained by single-particle electron cryo-microscopy reveals an amphipol belt replacing the membrane lipid bilayer. A precise fit of the X-ray structures of complex I, the complex III dimer, and monomeric complex IV indicates distances of 13 nm between the ubiquinol-binding sites of complexes I and III, and of 10-11 nm between the cytochrome c binding sites of complexes III and IV. The arrangement of respiratory chain complexes suggests two possible pathways for efficient electron transfer through the supercomplex, of which the shorter branch through the complex III monomer proximal to complex I may be preferred.

MeSH Terms
Animals Cattle Crystallography, X-Ray Electron Transport Electron Transport Chain Complex Proteins/metabolism Electron Transport Complex I/chemistry,metabolism Electron Transport Complex III/chemistry,metabolism Electron Transport Complex IV/chemistry,metabolism Mitochondria, Heart/metabolism Mitochondrial Membranes/enzymology,metabolism Multienzyme Complexes/chemistry,metabolism Oxidation-Reduction Protein Conformation Protein Multimerization Protein Structure, Quaternary
Chemicals
Electron Transport Chain Complex Proteins Multienzyme Complexes Electron Transport Complex IV Electron Transport Complex I Electron Transport Complex III
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Althoff Thorsten
Abteilung Strukturbiologie, Max-Planck-Institut für Biophysik, Frankfurt, Germany.
Mills Deryck J
Popot Jean-Luc
Kühlbrandt Werner
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2011.324
Published
2011-09-09
Epub
2011-00-09
Pages
4652-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC3243592
Subset
IM
Grants
NCRR NIH HHS · P41 RR001081 · United States
NCRR NIH HHS · P41 RR-01081 · United States
Databases
PDB
2YBB
External Links
PubMed source DOI: 10.1038/emboj.2011.324 PMC Full Text View journal details
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