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PMID: 15713802 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of a mitochondrial supercomplex formed by respiratory-chain complexes I and III.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 9 ·2005-03-01 ·Pages 3225-9

Dudkina NV, Eubel H, Keegstra W, Boekema EJ, Braun HP

Abstract

Mitochondria are central to the efficient provision of energy for eukaryotic cells. The oxidative-phosphorylation system of mitochondria consists of a series of five major membrane complexes: NADH-ubiquinone oxidoreductase (commonly known as complex I), succinate-ubiquinone oxidoreductase (complex II), ubiquinol-cytochrome c oxidoreductase (cytochrome bc1 complex or complex III), cytochrome c-O2 oxidoreductase (complex IV), and F1F0-ATP synthase (complex V). Several lines of evidence have recently suggested that complexes I and III-V might interact to form supercomplexes. However, because of their fragility, the structures of these supercomplexes are still unknown. A stable supercomplex consisting of complex I and dimeric complex III was purified from plant mitochondria. Structural characterization by single-particle EM indicates a specific type of interaction between monomeric complex I and dimeric complex III in a 1:1 ratio. We present a model for how complexes I and III are spatially organized within the I+III2 supercomplex.

MeSH Terms
Arabidopsis Electron Transport Electron Transport Complex I/chemistry,isolation & purification,metabolism,ultrastructure Electron Transport Complex III/chemistry,isolation & purification,metabolism,ultrastructure Electrophoresis, Polyacrylamide Gel Microscopy, Electron Models, Molecular Protein Conformation
Chemicals
Electron Transport Complex I Electron Transport Complex III
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dudkina Natalia V
Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Eubel Holger
Keegstra Wilko
Boekema Egbert J
Braun Hans-Peter
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0408870102
Published
2005-03-01
Epub
2005-00-15
Pages
3225-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC552927
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0408870102 PMC Full Text View journal details
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