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PMID: 19732833 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The structure of eukaryotic and prokaryotic complex I.

Journal of structural biology ·Vol. 169 ·No. 1 ·2010-01-00 ·Pages 81-8

Clason T, Ruiz T, Schägger H, Peng G, Zickermann V, Brandt U, Michel H, Radermacher M

Abstract

The structures of the NADH dehydrogenases from Bos taurus and Aquifex aeolicus have been determined by 3D electron microscopy, and have been analyzed in comparison with the previously determined structure of Complex I from Yarrowia lipolytica. The results show a clearly preserved domain structure in the peripheral arm of complex I, which is similar in the bacterial and eukaryotic complex. The membrane arms of both eukaryotic complexes show a similar shape but also significant differences in distinctive domains. One of the major protuberances observed in Y. lipolytica complex I appears missing in the bovine complex, while a protuberance not found in Y. lipolytica connects in bovine complex I a domain of the peripheral arm to the membrane arm. The structural similarities of the peripheral arm agree with the common functional principle of all complex Is. The differences seen in the membrane arm may indicate differences in the regulatory mechanism of the enzyme in different species.

MeSH Terms
Animals Bacteria/metabolism Cattle Electron Transport Complex I/chemistry,metabolism,ultrastructure Eukaryota Microscopy, Electron Models, Molecular NADH Dehydrogenase/chemistry,metabolism,ultrastructure Protein Structure, Tertiary Yarrowia/metabolism
Chemicals
NADH Dehydrogenase Electron Transport Complex I
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Clason T
University of Vermont, College of Medicine, Department of Molecular Physiology and Biophysics, Burlington, VT 05405, USA.
Ruiz T
Schägger H
Peng G
Zickermann V
Brandt U
Michel H
Radermacher M
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Article Info
Journal
Journal of structural biology
Abbr.
J Struct Biol
ISSN
1095-8657
DOI
10.1016/j.jsb.2009.08.017
Published
2010-01-00
Epub
2009-00-02
Pages
81-8
Language
English
Region
United States
NLM ID
9011206
PMCID
PMC3144259
Subset
IM
Grants
NIGMS NIH HHS · R01 GM068650 · United States
NIGMS NIH HHS · R01 GM069551-05 · United States
NIGMS NIH HHS · R01 GM069551 · United States
NIGMS NIH HHS · R01 GM078202-02 · United States
NIGMS NIH HHS · R01 GM068650-04 · United States
NIGMS NIH HHS · R01 GM078202 · United States
External Links
PubMed source DOI: 10.1016/j.jsb.2009.08.017 PMC Full Text View journal details
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