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PMID: 16621601 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The three-dimensional structure of complex I from Yarrowia lipolytica: a highly dynamic enzyme.

Journal of structural biology ·Vol. 154 ·No. 3 ·2006-06-00 ·Pages 269-79

Radermacher M, Ruiz T, Clason T, Benjamin S, Brandt U, Zickermann V

Abstract

The structure of complex I from Yarrowia lipolytica was determined by three-dimensional electron microscopy. A random conical data set was collected from deep stain embedded particles. More than 14000 image pairs were analyzed. Through extensive classification combined with three-dimensional reconstruction, it was possible for the first time to show a much more detailed substructure of the complex. The peripheral arm is subdivided in at least six domains. The membrane arm shows two major protrusions on its matrix facing side and exhibits a channel like feature on the side facing the cytoplasm. Structures resembling a tether connecting the subunits near the catalytic center with the protrusions of the membrane arm provide a second connection between matrix and membrane domain.

MeSH Terms
Cell Membrane/metabolism Computational Biology/methods Cytoplasm/metabolism Electron Transport Complex I/chemistry Image Processing, Computer-Assisted Microscopy, Electron Molecular Conformation Protein Conformation Protein Structure, Tertiary Radon/chemistry Yarrowia/enzymology
Chemicals
Electron Transport Complex I Radon
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Radermacher M
University of Vermont, College of Medicine, Department of Molecular Physiology and Biophysics, Burlington, VT, USA. Michael.Radermacher@uvm.edu
Ruiz T
Clason T
Benjamin S
Brandt U
Zickermann V
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Article Info
Journal
Journal of structural biology
Abbr.
J Struct Biol
ISSN
1047-8477
DOI
10.1016/j.jsb.2006.02.011
Email
michael.radermacher@uvm.edu
Published
2006-06-00
Epub
2006-00-24
Pages
269-79
Language
English
Region
United States
NLM ID
9011206
PMCID
PMC1764498
Subset
IM
Grants
NIGMS NIH HHS · R01 GM068650 · United States
NIGMS NIH HHS · R01 GM069551 · United States
External Links
PubMed source DOI: 10.1016/j.jsb.2006.02.011 PMC Full Text View journal details
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