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PMID: 21908690 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis HsfB1 and HsfB2b act as repressors of the expression of heat-inducible Hsfs but positively regulate the acquired thermotolerance.

Plant physiology ·Vol. 157 ·No. 3 ·2011-11-00 ·Pages 1243-54

Ikeda M, Mitsuda N, Ohme-Takagi M

Abstract

Many eukaryotes have from one to three heat shock factors (Hsfs), but plants have more than 20 Hsfs, designated class A, B, and C. Class A Hsfs are activators of transcription, but details of the roles of individual Hsfs have not been fully characterized. We show here that Arabidopsis (Arabidopsis thaliana) HsfB1 and HsfB2b, members of class B, are transcriptional repressors and negatively regulate the expression of heat-inducible Hsfs (HsfA2, HsfA7a, HsfB1, and HsfB2b) and several heat shock protein genes. In hsfb1 hsfb2b double mutant plants, the expression of a large number of heat-inducible genes was enhanced in the non-heat condition (23°C) and the plants exhibited slightly higher heat tolerance at 42°C than the wild type, similar to Pro35S:HsfA2 plants. In addition, under extended heat stress conditions, expression of the heat-inducible Hsf genes remained consistently higher in hsfb1 hsfb2b than in the wild type. These data indicate that HsfB1 and HsfB2b suppress the general heat shock response under non-heat-stress conditions and in the attenuating period. On the other hand, HsfB1 and HsfB2b appear to be necessary for the expression of heat stress-inducible heat shock protein genes under heat stress conditions, which is necessary for acquired thermotolerance. We show that the heat stress response is finely regulated by activation and repression activities of Hsfs in Arabidopsis.

MeSH Terms
Adaptation, Physiological/genetics Amino Acid Sequence Arabidopsis/genetics,physiology Arabidopsis Proteins/chemistry,genetics,metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Gene Expression Regulation, Plant Genes, Plant/genetics Heat Shock Transcription Factors Heat-Shock Proteins/chemistry,genetics,metabolism Heat-Shock Response/genetics Hot Temperature Hypocotyl/genetics,growth & development Models, Biological Molecular Sequence Data Phenotype Plant Proteins/chemistry,genetics,metabolism Promoter Regions, Genetic/genetics Protein Structure, Tertiary Repressor Proteins/chemistry,genetics,metabolism Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic Up-Regulation/genetics
Chemicals
Arabidopsis Proteins DNA-Binding Proteins HSFA2 protein, Arabidopsis HSFB1 protein, Arabidopsis Heat Shock Transcription Factors Heat-Shock Proteins HsfB2b protein, Arabidopsis Plant Proteins Repressor Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ikeda Miho
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan.
Mitsuda Nobutaka
Ohme-Takagi Masaru
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.111.179036
Published
2011-11-00
Epub
2011-00-09
Pages
1243-54
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC3252156
Subset
IM
Databases
GEO
GSE31888
External Links
PubMed source DOI: 10.1104/pp.111.179036 PMC Full Text View journal details
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