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PMID: 21908625 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The RNA polymerase omega factor RpoZ is regulated by PhoP and has an important role in antibiotic biosynthesis and morphological differentiation in Streptomyces coelicolor.

Applied and environmental microbiology ·Vol. 77 ·No. 21 ·2011-11-00 ·Pages 7586-94

Santos-Beneit F, Barriuso-Iglesias M, Fernández-Martínez LT, Martínez-Castro M, Sola-Landa A, Rodríguez-García A, Martín JF

Abstract

The RNA polymerase (RNAP) omega factor (ω) forms a complex with the α₂ββ' core of this enzyme in bacteria. We have characterized the rpoZ gene of Streptomyces coelicolor, which encodes a small protein (90 amino acids) identified as the omega factor. Deletion of the rpoZ gene resulted in strains with a slightly reduced growth rate, although they were still able to sporulate. The biosynthesis of actinorhodin and, particularly, that of undecylprodigiosin were drastically reduced in the ΔrpoZ strain, suggesting that expression of these secondary metabolite biosynthetic genes is dependent upon the presence of RpoZ in the RNAP complex. Complementation of the ΔrpoZ mutant with the wild-type rpoZ allele restored both phenotype and antibiotic production. Interestingly, the rpoZ gene contains a PHO box in its promoter region. DNA binding assays showed that the phosphate response regulator PhoP binds to such a region. Since luciferase reporter studies showed that rpoZ promoter activity was increased in a ΔphoP background, it can be concluded that rpoZ is controlled negatively by PhoP, thus connecting phosphate depletion regulation with antibiotic production and morphological differentiation in Streptomyces.

MeSH Terms
Anthraquinones/metabolism Anti-Bacterial Agents/biosynthesis Bacterial Proteins/metabolism DNA-Directed RNA Polymerases/metabolism Gene Deletion Gene Expression Regulation, Bacterial Genetic Complementation Test Prodigiosin/analogs & derivatives,biosynthesis Promoter Regions, Genetic Protein Binding Sigma Factor/biosynthesis,genetics Streptomyces coelicolor/genetics,growth & development
Chemicals
Anthraquinones Anti-Bacterial Agents Bacterial Proteins Sigma Factor PhoP protein, Bacteria undecylprodigiosin DNA-Directed RNA Polymerases actinorhodin Prodigiosin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Santos-Beneit Fernando
Institute of Biotechnology-INBIOTEC, Avda. Real 1, 24006 León, Spain.
Barriuso-Iglesias Mónica
Fernández-Martínez Lorena T
Martínez-Castro Miriam
Sola-Landa Alberto
Rodríguez-García Antonio
Martín Juan F
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
Published
2011-11-00
Epub
2011-00-09
Pages
7586-94
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC3209170
Subset
IM
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