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PMID: 19321498 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phosphate control over nitrogen metabolism in Streptomyces coelicolor: direct and indirect negative control of glnR, glnA, glnII and amtB expression by the response regulator PhoP.

Nucleic acids research ·Vol. 37 ·No. 10 ·2009-06-00 ·Pages 3230-42

Rodríguez-García A, Sola-Landa A, Apel K, Santos-Beneit F, Martín JF

Abstract

Bacterial growth requires equilibrated concentration of C, N and P sources. This work shows a phosphate control over the nitrogen metabolism in the model actinomycete Streptomyces coelicolor. Phosphate control of metabolism in Streptomyces is exerted by the two component system PhoR-PhoP. The response regulator PhoP binds to well-known PHO boxes composed of direct repeat units (DRus). PhoP binds to the glnR promoter, encoding the major nitrogen regulator as shown by EMSA studies, but not to the glnRII promoter under identical experimental conditions. PhoP also binds to the promoters of glnA and glnII encoding two glutamine synthetases, and to the promoter of the amtB-glnK-glnD operon, encoding an ammonium transporter and two putative nitrogen sensing/regulatory proteins. Footprinting analyses revealed that the PhoP-binding sequence overlaps the GlnR boxes in both glnA and glnII. 'Information theory' quantitative analyses of base conservation allowed us to establish the structure of the PhoP-binding regions in the glnR, glnA, glnII and amtB genes. Expression studies using luxAB as reporter showed that PhoP represses the above mentioned nitrogen metabolism genes. A mutant deleted in PhoP showed increased expression of the nitrogen metabolism genes. The possible conservation of phosphate control over nitrogen metabolism in other microorganisms is discussed.

MeSH Terms
Bacterial Proteins/genetics,metabolism Binding Sites Cation Transport Proteins/genetics Gene Expression Regulation, Bacterial Glutamate-Ammonia Ligase/genetics Nitrogen/metabolism Operator Regions, Genetic Operon Phosphates/metabolism Promoter Regions, Genetic Repressor Proteins/metabolism Streptomyces coelicolor/genetics,metabolism Trans-Activators/genetics,metabolism Transcription, Genetic
Chemicals
Bacterial Proteins Cation Transport Proteins GlnR protein, Streptomyces coelicolor Phosphates Repressor Proteins Trans-Activators PhoP protein, Bacteria glutamine synthetase I Glutamate-Ammonia Ligase Nitrogen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rodríguez-García Antonio
Instituto de Biotecnología de León, INBIOTEC, Parque Científico de León, Av. Real, 1, 24006, León, Spain.
Sola-Landa Alberto
Apel Kristian
Santos-Beneit Fernando
Martín Juan F
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkp162
Published
2009-06-00
Epub
2009-00-24
Pages
3230-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2691820
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkp162 PMC Full Text View journal details
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