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PMID: 12730372 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The two-component PhoR-PhoP system controls both primary metabolism and secondary metabolite biosynthesis in Streptomyces lividans.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 10 ·2003-05-13 ·Pages 6133-8

Sola-Landa A, Moura RS, Martín JF

Abstract

The biosynthesis of most secondary metabolites in different bacteria is strongly depressed by inorganic phosphate. The two-component phoR-phoP system of Streptomyces lividans has been cloned and characterized. PhoR showed all of the characteristics of the membrane-bound sensor proteins, whereas PhoP is a member of the DNA-binding OmpR family. Deletion mutants lacking phoP or phoR-phoP, were unable to grow in minimal medium at low phosphate concentration (10 microM). Growth was fully restored by complementation with the phoR-phoP genes. Both S. lividans DeltaphoP and DeltaphoR-phoP deletion mutants were unable to synthesize extracellular alkaline phosphatase (AP) as shown by immunodetection with anti-AP antibodies and by enzymatic analysis, suggesting that the PhoR-PhoP system is required for expression of the AP gene (phoA). Synthesis of AP was restored by complementation of the deletion mutants with phoR-phoP. The biosynthesis of two secondary metabolites, actinorhodin and undecylprodigiosin, was significantly increased in both solid and liquid medium in the DeltaphoP or DeltaphoR-phoP deletion mutants. Negative phosphate control of both secondary metabolites was restored by complementation with the phoR-phoP cluster. These results prove that expression of both phoA and genes implicated in the biosynthesis of secondary metabolites in S. lividans is regulated by a mechanism involving the two-component PhoR-PhoP system.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Genes, Bacterial Kinetics Molecular Sequence Data Phosphates/metabolism Plasmids Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid Streptomyces/genetics,growth & development,metabolism
Chemicals
Bacterial Proteins PhoQ protein, Bacteria Phosphates PhoR protein, Bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sola-Landa A
Instituto de Biotecnologia de León (INBIOTEC), Parque Cientifico de León, Avenida del Real, n degrees 1, Spain.
Moura R S
Martín J F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0931429100
Published
2003-05-13
Epub
2003-00-02
Pages
6133-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC156338
Subset
IM
Databases
GENBANK
AJ544582
External Links
PubMed source DOI: 10.1073/pnas.0931429100 PMC Full Text View journal details
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