Luciferase detection during stationary phase in Lactococcus lactis.

Applied and environmental microbiology ·Vol. 73 ·No. 14 ·2007-07-00 ·Pages 4704-6

Bachmann H, Santos F, Kleerebezem M, van Hylckama Vlieg JE

Abstract

The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved by the addition of riboflavin or flavin mononucleotide to whole-cell assay systems.

MeSH Terms

Flavin Mononucleotide/metabolism Lactococcus lactis/enzymology,growth & development Luciferases/analysis Riboflavin/metabolism

Chemicals

Flavin Mononucleotide Luciferases Riboflavin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bachmann Herwig

NIZO food research, Kluyver Centre for Genomics of Industrial Fermentation, 6710 BA Ede, The Netherlands.

Santos Filipe

Kleerebezem Michiel

van Hylckama Vlieg Johan E T

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/AEM.02807-06

Published

2007-07-00

Epub

2007-00-18

Pages

4704-6

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC1932811

Subset

External Links

PubMed source DOI: 10.1128/AEM.02807-06 PMC Full Text View journal details

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Luciferase detection during stationary phase in Lactococcus lactis.

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