Home LiteratureArticle Details
PMID: 2185469 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a mammalian nuclear factor and human cDNA-encoded proteins that recognize DNA containing apurinic sites.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 9 ·1990-05-00 ·Pages 3396-400

Lenz J, Okenquist SA, LoSardo JE, Hamilton KK, Doetsch PW

Abstract

Damage to DNA can have lethal or mutagenic consequences for cells unless it is detected and repaired by cellular proteins. Repair depends on the ability of cellular factors to distinguish the damaged sites. Electrophoretic binding assays were used to identify a factor from the nuclei of mammalian cells that bound to DNA containing apurinic sites. A binding assay based on the use of beta-galactosidase fusion proteins was subsequently used to isolate recombinant clones of human cDNAs that encoded apurinic DNA-binding proteins. Two distinct human cDNAs were identified that encoded proteins that bound apurinic DNA preferentially over undamaged, methylated, or UV-irradiated DNA. These approaches may offer a general method for the detection of proteins that recognize various types of DNA damage and for the cloning of genes encoding such proteins.

MeSH Terms
Animals Apurinic Acid/metabolism Blotting, Southern Cell Line Cell Nucleus/metabolism Cloning, Molecular DNA Probes DNA, Recombinant/isolation & purification DNA-Binding Proteins/genetics Escherichia coli/genetics Nuclear Proteins/genetics Substrate Specificity
Chemicals
DNA Probes DNA, Recombinant DNA-Binding Proteins Nuclear Proteins Apurinic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lenz J
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461.
Okenquist S A
LoSardo J E
Hamilton K K
Doetsch P W
References (37)
37 references, click to expand
  1. Rate of depurination of native deoxyribonucleic acid.
    Biochemistry. 1972 Sep 12;11(19):3610-8 PMID: 4626532
  2. Isolation of cDNAs encoding a human protein that binds selectively to DNA modified by the anticancer drug cis-diamminedichloroplatinum(II)
    Proc Natl Acad Sci U S A. 1989 Nov;86(21):8328-32 PMID: 2530581
  3. Human endonuclease specific for apurinic/apyrimidinic sites in DNA. Partial purification and characterization of multiple forms from placenta.
    J Biol Chem. 1977 Feb 25;252(4):1235-42 PMID: 14146
  4. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  5. Xeroderma pigmentosum fibroblasts of the D group lack an apurinic DNA endonuclease species with a low apparent Km.
    Nucleic Acids Res. 1978 Mar;5(3):951-60 PMID: 643622
  6. DNA glycosylases, endonucleases for apurinic/apyrimidinic sites, and base excision-repair.
    Prog Nucleic Acid Res Mol Biol. 1979;22:135-92 PMID: 392601
  7. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  8. Depurination causes mutations in SOS-induced cells.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1773-7 PMID: 6453349
  9. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
    Nucleic Acids Res. 1981 Jul 10;9(13):3047-60 PMID: 6269071
  10. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  11. Human placental apurinic/apyrimidinic endonuclease. Its isolation and characterization.
    J Biol Chem. 1982 Nov 25;257(22):13455-8 PMID: 7142159
  12. Human placental apurinic/apyrimidinic endonuclease. Mechanism of action.
    J Biol Chem. 1982 Nov 25;257(22):13459-64 PMID: 6183261
  13. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  14. Properties of a human lymphoblast AP-endonuclease associated with activity for DNA damaged by ultraviolet light, gamma-rays, or osmium tetroxide.
    Biochemistry. 1983 Sep 13;22(19):4507-12 PMID: 6578848
  15. Determination of the leukaemogenicity of a murine retrovirus by sequences within the long terminal repeat.
    Nature. 1984 Mar 29-Apr 4;308(5958):467-70 PMID: 6323995
  16. Mutational specificity of depurination.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1494-8 PMID: 6369329
  17. Apurinic sites cause mutations in simian virus 40.
    Mutat Res. 1984 Nov;129(2):141-7 PMID: 6095075
  18. Apurinic sites as mutagenic intermediates.
    Cell. 1985 Mar;40(3):483-4 PMID: 2982494
  19. Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):6741-4 PMID: 2995982
  20. Comparison of apurinic DNA-binding protein from an ataxia telangiectasia and a HeLa cell line. Evidence for an altered processing of apurinic/apyrimidinic endonuclease.
    J Biol Chem. 1985 Dec 5;260(28):14918-24 PMID: 2415510
  21. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
  22. A yeast gene that is essential for release from glucose repression encodes a protein kinase.
    Science. 1986 Sep 12;233(4769):1175-80 PMID: 3526554
  23. Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer.
    Mol Cell Biol. 1987 Mar;7(3):1101-10 PMID: 3561410
  24. Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA.
    Cell. 1988 Feb 12;52(3):415-23 PMID: 2964277
  25. Yeast redoxyendonuclease, a DNA repair enzyme similar to Escherichia coli endonuclease III.
    Biochemistry. 1988 Apr 5;27(7):2629-34 PMID: 2454659
  26. Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif.
    Science. 1988 Jul 29;241(4865):577-80 PMID: 3399892
  27. Xeroderma pigmentosum group E cells lack a nuclear factor that binds to damaged DNA.
    Science. 1988 Oct 28;242(4878):564-7 PMID: 3175673
  28. DNA repair enzymes.
    Annu Rev Biochem. 1988;57:29-67 PMID: 3052275
  29. A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotype.
    Cell. 1988 Nov 4;55(3):519-29 PMID: 2902928
  30. Identification of the SL3-3 virus enhancer core as a T-lymphoma cell-specific element.
    J Virol. 1989 Jan;63(1):76-84 PMID: 2535754
  31. Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
    Science. 1988 Dec 9;242(4884):1430-3 PMID: 2974179
  32. In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
    Genes Dev. 1988 Jul;2(7):801-6 PMID: 3061875
  33. The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
    Genes Dev. 1988 Dec;2(12A):1582-99 PMID: 2905684
  34. Differences in activities of murine retroviral long terminal repeats in cytotoxic T lymphocytes and T-lymphoma cells.
    J Virol. 1989 Mar;63(3):1087-94 PMID: 2644446
  35. UV-induced DNA-binding proteins in human cells.
    Proc Natl Acad Sci U S A. 1989 Feb;86(4):1163-7 PMID: 2919165
  36. Partial purification and characterization of an endonuclease from spinach that cleaves ultraviolet light-damaged duplex DNA.
    Biochim Biophys Acta. 1989 Apr 12;1007(3):309-17 PMID: 2495026
  37. An altered apurinic DNA endonuclease activity in group A and group D xeroderma pigmentosum fibroblasts.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1169-73 PMID: 1063398
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.9.3396
Published
1990-05-00
Pages
3396-400
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53907
Subset
IM
Grants
NCI NIH HHS · CA01441 · United States
NCI NIH HHS · CA31855 · United States
NCI NIH HHS · CA42607 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.9.3396 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2