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PMID: 21836625 Published · epublish English Journal Article Research Support, N.I.H., Extramural Review

The DNA-packaging nanomotor of tailed bacteriophages.

Nature reviews. Microbiology ·Vol. 9 ·No. 9 ·2011-08-12 ·Pages 647-57

Casjens SR

Abstract

Tailed bacteriophages use nanomotors, or molecular machines that convert chemical energy into physical movement of molecules, to insert their double-stranded DNA genomes into virus particles. These viral nanomotors are powered by ATP hydrolysis and pump the DNA into a preformed protein container called a procapsid. As a result, the virions contain very highly compacted chromosomes. Here, I review recent progress in obtaining structural information for virions, procapsids and the individual motor protein components, and discuss single-molecule in vitro packaging reactions, which have yielded important new information about the mechanism by which these powerful molecular machines translocate DNA.

MeSH Terms
Adenosine Triphosphate/physiology Bacteriophages/physiology,ultrastructure Capsid/physiology,ultrastructure Cryoelectron Microscopy DNA Packaging/physiology DNA, Viral/physiology Molecular Motor Proteins/physiology
Chemicals
DNA, Viral Molecular Motor Proteins Adenosine Triphosphate
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Casjens Sherwood R
Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, 15 North Medical Drive East, Salt Lake City, Utah 84112, USA. sherwood.casjens@path.utah.edu
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Article Info
Journal
Nature reviews. Microbiology
Abbr.
Nat Rev Microbiol
ISSN
1740-1534
DOI
10.1038/nrmicro2632
Email
sherwood.casjens@path.utah.edu
Published
2011-08-12
Epub
2011-00-12
Pages
647-57
Language
English
Region
England
NLM ID
101190261
Subset
IM
Grants
NIAID NIH HHS · R01 AI074825 · United States
External Links
PubMed source DOI: 10.1038/nrmicro2632 View journal details
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