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PMID: 2170677 Published · ppublish English Journal Article

A region of the 5' noncoding region of foot-and-mouth disease virus RNA directs efficient internal initiation of protein synthesis within cells: involvement with the role of L protease in translational control.

Journal of virology ·Vol. 64 ·No. 11 ·1990-11-00 ·Pages 5389-95

Belsham GJ, Brangwyn JK

Abstract

Plasmids encoding bicistronic mRNAs have been constructed and used to identify a region from the 5' noncoding region of foot-and-mouth disease virus (FMDV) which directs efficient internal initiation of protein synthesis within cells. The loss of about 30 nucleotides (nt) from the 5' terminus or about 50 nt from the 3' terminus of the 435-nt region completely abolished the activity of this region. The expression of the FMDV L protease severely inhibited the expression of other genes unless they were preceded by this element. The regulation of protein synthesis mediated by FMDV is discussed.

MeSH Terms
Animals Aphthovirus/enzymology,genetics Base Sequence Binding Sites Carrier Proteins/metabolism Cell Line Endopeptidases/physiology Gene Expression Regulation, Viral Genes In Vitro Techniques Molecular Sequence Data Oligonucleotides Peptide Chain Initiation, Translational Plasmids RNA Cap-Binding Proteins RNA, Viral/genetics Regulatory Sequences, Nucleic Acid Ribosomes/metabolism
Chemicals
Carrier Proteins Oligonucleotides RNA Cap-Binding Proteins RNA, Viral Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Belsham G J
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, United Kingdom.
Brangwyn J K
References (26)
26 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Intracellular expression and processing of foot-and-mouth disease virus capsid precursors using vaccinia virus vectors: influence of the L protease.
    Virology. 1990 Jun;176(2):524-30 PMID: 2161149
  3. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  4. Nucleotide sequence and genome organization of foot-and-mouth disease virus.
    Nucleic Acids Res. 1984 Aug 24;12(16):6587-601 PMID: 6089122
  5. Two initiation sites for foot-and-mouth disease virus polyprotein in vivo.
    J Gen Virol. 1985 Dec;66 ( Pt 12):2615-26 PMID: 2999308
  6. Cleavage of the cap binding protein complex polypeptide p220 is not effected by the second poliovirus protease 2A.
    Virology. 1986 Apr 15;150(1):299-303 PMID: 3006342
  7. The sequence of foot-and-mouth disease virus RNA to the 5' side of the poly(C) tract.
    Gene. 1985;40(2-3):331-6 PMID: 3007298
  8. A second protease of foot-and-mouth disease virus.
    J Virol. 1986 Jun;58(3):893-9 PMID: 3009894
  9. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8122-6 PMID: 3095828
  10. Proteolysis of the p220 component of the cap-binding protein complex is not sufficient for complete inhibition of host cell protein synthesis after poliovirus infection.
    J Virol. 1987 Apr;61(4):986-91 PMID: 3029432
  11. Effects of cDNA hybridization on translation of encephalomyocarditis virus RNA.
    J Virol. 1987 Jun;61(6):2033-7 PMID: 3033330
  12. Potential secondary and tertiary structure in the genomic RNA of foot and mouth disease virus.
    Nucleic Acids Res. 1987 Sep 11;15(17):7067-79 PMID: 2821491
  13. Studies on the infectivity of foot-and-mouth disease virus RNA using microinjection.
    J Gen Virol. 1988 Feb;69 ( Pt 2):265-74 PMID: 2448416
  14. A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation.
    J Virol. 1988 Aug;62(8):2636-43 PMID: 2839690
  15. RNA translation. Picornaviruses break the rules.
    Nature. 1988 Jul 28;334(6180):292-3 PMID: 3393222
  16. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA.
    Nature. 1988 Jul 28;334(6180):320-5 PMID: 2839775
  17. Relationship of p220 cleavage during picornavirus infection to 2A proteinase sequencing.
    J Virol. 1988 Nov;62(11):4216-23 PMID: 2845133
  18. Leader protein of foot-and-mouth disease virus is required for cleavage of the p220 component of the cap-binding protein complex.
    J Virol. 1988 Nov;62(11):4407-9 PMID: 2845152
  19. The scanning model for translation: an update.
    J Cell Biol. 1989 Feb;108(2):229-41 PMID: 2645293
  20. Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo.
    J Virol. 1989 Apr;63(4):1651-60 PMID: 2538648
  21. Structure and stability of mRNA synthesized by vaccinia virus-encoded bacteriophage T7 RNA polymerase in mammalian cells. Importance of the 5' untranslated leader.
    J Mol Biol. 1989 Mar 20;206(2):333-48 PMID: 2497259
  22. Use of modified T7 DNA polymerase (sequenase version 2.0) for oligonucleotide site-directed mutagenesis.
    Nucleic Acids Res. 1989 Apr 25;17(8):3314 PMID: 2726477
  23. Conservation of the secondary structure elements of the 5'-untranslated region of cardio- and aphthovirus RNAs.
    Nucleic Acids Res. 1989 Jul 25;17(14):5701-11 PMID: 2548167
  24. Interference with vaccinia virus growth caused by insertion of the coding sequence for poliovirus protease 2A.
    Virology. 1989 Dec;173(2):509-21 PMID: 2556841
  25. Limited expression of poliovirus by vaccinia virus recombinants due to inhibition of the vector by proteinase 2A.
    J Virol. 1990 Mar;64(3):1388-93 PMID: 2154618
  26. Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.
    J Biol Chem. 1982 Dec 25;257(24):14806-10 PMID: 6294080
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.11.5389-5395.1990
Published
1990-11-00
Pages
5389-95
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248589
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.11.5389-5395.1990 PMC Full Text View journal details
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