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PMID: 2154618 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Limited expression of poliovirus by vaccinia virus recombinants due to inhibition of the vector by proteinase 2A.

Journal of virology ·Vol. 64 ·No. 3 ·1990-03-00 ·Pages 1388-93

Jewell JE, Ball LA, Rueckert R

Abstract

A recombinant vaccinia virus was constructed that expressed poliovirus coat precursor protein P1 fused to about two-thirds of the 2A proteinase. The truncated 2A segment could be cleaved away from the P1 region by coinfecting with poliovirus type 1, 2, or 3 or with human rhinovirus 14 but not with encephalomyocarditis virus. Further cleavage of the vector-derived P1 to yield mature poliovirus capsid proteins was not observed. Attempts to isolate vaccinia virus recombinants containing portions of the poliovirus genome that encompassed the complete gene for proteinase 2A were unsuccessful, unless expression of functional 2A was abolished by insertion of a frameshift mutation. We conclude that an activity of the 2A proteinase, probably its role in translational inhibition, prevented isolation of vaccinia virus recombinants that expressed 2A.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Capsid/genetics,metabolism Capsid Proteins Cell Line Cysteine Endopeptidases/genetics,metabolism Genes, Viral Genetic Vectors HeLa Cells/metabolism Humans Molecular Sequence Data Plasmids Poliovirus/genetics Recombinant Fusion Proteins/metabolism Restriction Mapping Transfection Vaccinia virus/genetics Viral Proteins
Chemicals
Capsid Proteins Recombinant Fusion Proteins VP1 protein, Poliovirus Viral Proteins Cysteine Endopeptidases picornain 2A, Picornavirus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jewell J E
Institute for Molecular Virology, University of Wisconsin, Madison 53706.
Ball L A
Rueckert R
References (34)
34 references, click to expand
  1. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  2. A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1777-81 PMID: 3006071
  3. 5'-terminal structure of poliovirus polyribosomal RNA is pUp.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):327-30 PMID: 174102
  4. The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):375-80 PMID: 174106
  5. Protease required for processing picornaviral coat protein resides in the viral replicase gene.
    J Virol. 1979 Dec;32(3):770-8 PMID: 229266
  6. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887-91 PMID: 6272282
  7. Proteolytic processing of poliovirus polypeptides: antibodies to polypeptide P3-7c inhibit cleavage at glutamine-glycine pairs.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):3973-7 PMID: 6287457
  8. Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.
    J Biol Chem. 1982 Dec 25;257(24):14806-10 PMID: 6294080
  9. Vaccinia virus: a selectable eukaryotic cloning and expression vector.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415-9 PMID: 6296831
  10. Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen.
    Nature. 1983 Apr 7;302(5908):490-5 PMID: 6835382
  11. Nucleotide sequence of the vaccinia virus thymidine kinase gene and the nature of spontaneous frameshift mutations.
    J Virol. 1983 May;46(2):530-7 PMID: 6842679
  12. Quick-blot: selective mRNA or DNA immobilization from whole cells.
    DNA. 1983;2(3):243-54 PMID: 6641475
  13. Infectious poxvirus vectors have capacity for at least 25 000 base pairs of foreign DNA.
    Gene. 1983 Nov;25(1):21-8 PMID: 6229451
  14. Construction of live vaccines using genetically engineered poxviruses: biological activity of vaccinia virus recombinants expressing the hepatitis B virus surface antigen and the herpes simplex virus glycoprotein D.
    Proc Natl Acad Sci U S A. 1984 Jan;81(1):193-7 PMID: 6320164
  15. General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes.
    J Virol. 1984 Mar;49(3):857-64 PMID: 6321770
  16. The nucleotide and deduced amino acid sequences of the encephalomyocarditis viral polyprotein coding region.
    Nucleic Acids Res. 1984 Mar 26;12(6):2969-85 PMID: 6324136
  17. Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.
    Nucleic Acids Res. 1984 Jun 25;12(12):5123-41 PMID: 6330698
  18. In vitro mutagenesis of the promoter region for a vaccinia virus gene: evidence for tandem early and late regulatory signals.
    J Virol. 1985 Apr;54(1):30-7 PMID: 3973982
  19. Molecular cloning and complete sequence determination of RNA genome of human rhinovirus type 14.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):732-6 PMID: 2983312
  20. One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):2096-100 PMID: 3856886
  21. Poliovirus protease does not mediate cleavage of the 220,000-Da component of the cap binding protein complex.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2723-7 PMID: 2986132
  22. Expression of the major glycoprotein G of human respiratory syncytial virus from recombinant vaccinia virus vectors.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):246-50 PMID: 3455762
  23. Cleavage of the cap binding protein complex polypeptide p220 is not effected by the second poliovirus protease 2A.
    Virology. 1986 Apr 15;150(1):299-303 PMID: 3006342
  24. A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein.
    Cell. 1986 Jun 6;45(5):761-70 PMID: 3011278
  25. Encephalomyocarditis virus 3C protease: efficient cell-free expression from clones which link viral 5' noncoding sequences to the P3 region.
    J Virol. 1986 Nov;60(2):376-84 PMID: 3021972
  26. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8122-6 PMID: 3095828
  27. Poliovirus polypeptide precursors: expression in vitro and processing by exogenous 3C and 2A proteinases.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):4002-6 PMID: 3035560
  28. Poliovirus proteinase 2A induces cleavage of eucaryotic initiation factor 4F polypeptide p220.
    J Virol. 1987 Sep;61(9):2711-8 PMID: 3039165
  29. Purification and partial characterization of poliovirus protease 2A by means of a functional assay.
    J Virol. 1988 Apr;62(4):1243-50 PMID: 2831385
  30. Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7872-6 PMID: 3186696
  31. Human immunodeficiency virus tat-activated expression of poliovirus protein 2A inhibits mRNA translation.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2143-6 PMID: 2538833
  32. Transfer of the inducible lac repressor/operator system from Escherichia coli to a vaccinia virus expression vector.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2549-53 PMID: 2649884
  33. The purification fo four strains of poxvirus.
    Virology. 1962 Sep;18:9-18 PMID: 14036977
  34. Non-producer human cells induced by murine sarcoma virus.
    Int J Cancer. 1975 Jan 15;15(1):23-9 PMID: 165148
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.3.1388-1393.1990
Published
1990-03-00
Pages
1388-93
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249263
Subset
IM
Grants
NIAID NIH HHS · AI-18270 · United States
NIAID NIH HHS · AI-24939 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.3.1388-1393.1990 PMC Full Text View journal details
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