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PMID: 2167294 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Conservation of Salmonella typhimurium virulence plasmid maintenance regions among Salmonella serovars as a basis for plasmid curing.

Infection and immunity ·Vol. 58 ·No. 9 ·1990-09-00 ·Pages 3084-92

Tinge SA, Curtiss R

Abstract

The association of large plasmids with virulence in invasive Salmonella serovars has led to a number of studies designed to uncover the role of these plasmids in virulence. This study addresses two aspects of virulence-associated plasmids. The first is the distribution of the replication and maintenance regions among the plasmids of different Salmonella serovars, and the second is the use of the conserved virulence plasmid par region to provide a rapid method for eliminating the virulence plasmids specifically. Colony blots revealed that the par and repB regions of the S. typhimurium virulence plasmid hybridized with 80% of the isolates of S. choleraesuis, S. dublin, S. enteritidis, S. gallinarum, S. pullorum, and S. typhimurium, while the repC region was not detected in any of the isolates of S. dublin, S. gallinarum, or S. pullorum. None of these maintenance regions was found in any of the 30 additional serovars tested. The large plasmids of those serovars that hybridized with par were labeled with a Kmr insert within parA via P22HTint or P1L4 transduction, which destabilized the plasmids and allowed the rapid isolation of plasmid-free derivatives for all of the serovars, except for S. dublin, which exhibited weak homology with par.

MeSH Terms
Base Sequence Chromosome Mapping DNA Transposable Elements Gene Amplification Nucleic Acid Hybridization Plasmids/genetics Replicon Salmonella typhimurium/genetics,pathogenicity Sequence Homology, Nucleic Acid Transduction, Genetic Virulence/genetics
Chemicals
DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tinge S A
Department of Biology, Washington University, St. Louis, Missouri 63130.
Curtiss R
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41 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.9.3084-3092.1990
Published
1990-09-00
Pages
3084-92
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313615
Subset
IM
Grants
NIAID NIH HHS · AI24533 · United States
NIDCR NIH HHS · DE06669 · United States
External Links
PubMed source DOI: 10.1128/iai.58.9.3084-3092.1990 PMC Full Text View journal details
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