Abstract
Cells containing a deletion of either the SCR1 or SCR2 genes, which code for the 7SL RNA component of the signal recognition particle (SRP) homologue, were found to be viable. Two independent approaches demonstrated that cells containing deletions of both genes were inviable. Therefore, Yarrowia lipolytica contains two (and only two) functional 7SL RNA genes.
MeSH Terms
Blotting, Southern
Chromosome Deletion
Genes, Fungal
RNA, Fungal/genetics
Restriction Mapping
Ribonucleoproteins/genetics
Saccharomycetales/genetics,growth & development
Signal Recognition Particle
Transformation, Genetic
Chemicals
RNA, Fungal
Ribonucleoproteins
Signal Recognition Particle
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
He F
Laboratoire de Genetique, Centre de Biotechnologie Agro-Industrielle, INAPGt, Thiverval Grignon, France.
Yaver D
Beckerich J M
Ogrydziak D
Gaillardin C
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