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PMID: 2657742 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic analysis of Schizosaccharomyces pombe 7SL RNA: a structural motif that includes a conserved tetranucleotide loop is important for function.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 11 ·1989-06-00 ·Pages 4137-41

Liao XB, Brennwald P, Wise JA

Abstract

We have studied the effects of mutations in a 6-base segment of Schizosaccharomyces pombe 7SL RNA, which lies within a 35-nucleotide domain whose sequence and secondary structure are conserved in RNAs from many divergent organisms, including the 7SL component of human signal recognition particle (SRP). Surprisingly, many changes in this region can be tolerated under normal growth conditions. An exception is the lethality of several mutations at positions 159 and 160, 2 nucleotides previously shown to be protected from RNase digestion by the 19-kDa canine SRP protein. Nucleotide 160 is, in addition, the most highly conserved base in a consensus sequence for the most common tetranucleotide loop in ribosomal RNAs. Mutations that are likely to affect the stability and/or conformation of the RNA give rise to a conditional phenotype: when osmolarity of the medium is raised, the RNAs become partially or completely defective in function at high temperature.

MeSH Terms
Base Sequence Escherichia coli/genetics Genetic Complementation Test Molecular Sequence Data Mutation Nucleic Acid Conformation Plasmids RNA, Fungal/genetics Saccharomycetales/genetics Schizosaccharomyces/genetics Transformation, Genetic
Chemicals
RNA, Fungal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liao X B
Department of Biochemistry, University of Illinois, Urbana 61801.
Brennwald P
Wise J A
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.11.4137
Published
1989-06-00
Pages
4137-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287404
Subset
IM
Grants
NIGMS NIH HHS · 1 RO1 GM 34891 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.11.4137 PMC Full Text View journal details
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